This study aimed to select fermentation conditions able simultaneously maximize the production of lipids and carotenoids by oleaginous yeast cultivated in wheat straw hydrolysate. An evolved strain Rhodosporidium toruloides with improved tolerance toxic compounds present hydrolysate medium was used. Experiments were performed order investigate effect temperature inoculum load on R. toruloides. ...

BACKGROUND Rhodosporidium and Rhodotorula are two genera of oleaginous red yeast with great potential for industrial biotechnology. To date, there is no effective method for inducible expression of proteins and RNAs in these hosts. RESULTS We have developed a luciferase gene reporter assay based on a new codon-optimized LUC2 reporter gene (RtLUC2), which is flanked with CAR2 homology arms and...