More than 70 different proteins form two subunits of eukaryotic ribosomes and are major components of the total cellular protein. It has been reported that in various living organisms the biosynthesis of the different ribosomal proteins is regulated coordinately (Mager, 1988). Although some information concerning the primary structure of plant ribosoma1 proteins is available by isolating cDNA c...

Total protein was released from isolated HeLa cell nucleoli by guanidine hydrochloride, purified by cesium chloride density gradient centrifugation, and analyzed by two-dimensional polyacrylamide gel electrophoresis. Conditions of electrophoresis restricted attention to proteins that are positively charged at pH 8.6. Most of the major nucleolar protein spots co-electrophoresed with ribosomal pr...

Synthetic biology constructs often rely upon the introduction of “circuit” genes into host cells, in order to express novel proteins and thus endow with a desired behavior. The expression these new “consumes” existing resources cell, such as ATP, RNA polymerase, amino acids, ribosomes. Ribosomal competition among strands mRNA may be described by system nonlinear ODEs called Flow Model (RFM). fo...

Total protein was released from isolated HeLa cell nucleoli by guanidine hydrochloride, purified by cesium chloride density gradient centrifugation, and analyzed by two-dimensional polyacrylamide gel electrophoresis. Conditions of electrophoresis restricted attention to proteins that are positively charged at pH 8.6. Most of the major nucleolar protein spots co-electrophoresed with ribosomal pr...

A significant percentage of the methyltransferasome in Saccharomyces cerevisiae and higher eukaryotes is devoted to methylation of the translational machinery. Methylation of the RNA components of the translational machinery has been studied extensively and is important for structure stability, ribosome biogenesis, and translational fidelity. However, the functional effects of ribosomal protein...