Background and Aims: Avian metapneumovirus (aMPV) is RNA virus responsible for upper respiratory disease in poultry which is usually accompanied by secondary bacterial infections. In Iran, some molecular and serological studies indicated that aMPV infection occurred in commercial poultry industry. Materials and Methods: In this study aMPV was molecularly identified and characterized in an affec...

Background: Hepatitis C virus (HCV) infection is the most common transfusion transmitted disease in poly-transfused patients worldwide. In this study we aimed to evaluate the effects of pegylated interferon alfa-2a (PEG-IFN A-2a) in reducing serum ALT and eradicating serum hepatitis C virus (HCV) RNA in HCV infected polytransfused thalassemic patients. Materials and Methods: A cohort of 51 HCV-...

background and objectives: hepatitis c virus (hcv) is a major public health problem worldwide. replication and persistence of hcv genome have been described in the liver tissue as well as b cells lymphocyte. several investigations have reported that long-term persistence of hcv in b cells may result in hodgkin and non-hodgkin lymphoma. this study was aimed to determine frequency of hcv rna in h...

Nowadays viral hepatitis is one of the most common and important causes of chronic liver disorders in the world. Extrahepatic manifestations of viral hepatitis consist a main part of the disease. The main viruses causing hepatitis include Hepatitis A, B and C viruses. Herein common skin manifestations in hepatitis C virus (HCV) are discussed. Chronic hepatitis causes skin disorders both directl...

Translation and replication of positive stranded RNA viruses are directly initiated in the cellular cytoplasm after uncoating of the viral genome. Accordingly, infectious virus can be generated by transfection of RNA genomes into susceptible cells. In the present study, efficiency of conventional virus isolation after inoculation of cells with infectious sample material was compared to virus re...

Abstract: Background  and  Aims:  Huh-7  is  a  cell  line  that  was  derived  from  a  liver  tumor  of  a  Japanese  man.  Hepatocellular  carcinoma  (HCC)  is  considered  as  a  primary  liver  cancer.  Highly  resistant  tumor  to  treatment  which  causes  the  death  of  many  patients  annually.  Thus,  targeting  the  cancer  cells  by  using  a  new  method  could  be  effective  in...

Purified virions of Aura virus, a South American alphavirus related to Sindbis virus, were found to contain two RNA species, one of 12 kb and the other of 4.2 kb. Northern (RNA) blot analysis, primer extension analysis, and limited sequencing showed that the 12-kb RNA was the viral genomic RNA, whereas the 4.2-kb RNA present in virus preparations was identical to the 26S subgenomic RNA present ...

Abstract Favipiravir is a modern antiviral drug that can be used in developing viral pandemics, such as the Ebola virus, H1N1 flu, Lassa fever, and haemorrhagic fever outbreaks Argentina 2009. A. Chain conclusion transformed to favipiravir-RTP inserted into RNA strand elongating. Then, since favipiravir acts chain terminator, elongation slows down at incorporation site of does not continue. The...

Rous sarcoma virus harvested from cells at intervals of 3 min has the same density, sedimentation coefficient, and DNA polymerase as virus harvested at hourly intervals. The RNA of the Prague strain-C consists of a minor class of 60-70S RNA, a major class 30-40S RNA, and a 4-12S class of RNA present at variable concentration. The RNA of the Schmidt-Ruppin strain-A contains more 60-70S than 30-4...