The pandemic of coronavirus disease (COVID-19) has caused serious adverse effects on the human race. There are limited data maternal and neonatal outcomes pregnant women with COVID-19. An observational descriptive study was conducted in department obstetrics & gynaecology at SMSR Sharda hospital located Greater Noida, Uttar Pradesh. This medical college drains patients from entire Noida as ...

The full coding sequence for porcine erythropoietin receptor (EPOR) was elucidated using reverse transcription polymerase chain reaction (PCR) (rtPCR) and 3' and 5' rapid amplification of cDNA ends (RACE) procedures. Total RNA collected from Day 30 fetal liver was used as starting material. A 1843 bp sequence was obtained from which could be inferred a 509 amino acid protein which was 79-85% id...

By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RTPCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transien...

In July 2001, a great egret (Casmerodius albus) was found dead in Charlton County, Georgia (USA) and submitted to the Southeastern Cooperative Wildlife Disease Study (The University of Georgia, Athens, Georgia). Histopathologic findings included severe hepatic necrosis and necrosis of sheathed arterioles. Eastern equine encephalitis (EEE) virus was isolated from brain and heart using Vero cells...

A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...

Objective: DLBS3233 is a standardized extract combination containing Lagerstroemia speciosa and Cinnamomum burmannii. The effect of DLBS3233 on adipocyte differentiation was examined in this study. Methods: 3T3-L1 pre-adipocyte was used to investigate gene expression using the real-time reverse transcription polymerase chain reaction (RTPCR) method. Oil red-O staining for detecting lipid format...

Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of ...

Combining reverse transcriptase-polymerase chain reaction (RTPCR) and molecular cloning technologies, we have investigated the expression of homeodomain proteins in chicken gizzard tissues undergoing differentiation. We demonstrate that homeodomain encoding transcripts are found in developing chicken gizzard. An RT-PCR generated 117 bp homeodomain DNA probe was used to screen a gizzard library ...

A new cucumber mosaic virus (CMV) subgroup based on recent sequence data has been proposed by Palukaitis and Zaitlin (1997) to distinguish between some strains in the former subgroup I. The three subgroups are tentatively named IA, IB and II. Here we describe a simple and rapid procedure based on the reverse transcriptase-polymerase chain reaction (RTPCR) followed by enzymatic digestion (RFLP) ...