Ruminally undegraded protein (RUP) values of blood meal (n = 2), hydrolyzed feather meal (n = 2), fish meal (n = 2), meat and bone meal, and soybean meal were estimated using an in situ method, an inhibitor in vitro method, and an inhibitor in vitro technique applying Michaelis-Menten saturation kinetics. Degradation rates for in situ and inhibitor in vitro methods were calculated by regression...

The effects of altering forage particle size and source of rapidly degradable carbohydrates on in situ degradation and ruminal variables were studied in four Iranian male sheep. The study was designed as a Latin square with a 2 × 2 factorial arrangement of treatments including two carbohydrate sources (pelleted beet pulp vs. maizeand barley-based concentrate) and two lucerne particle sizes (2.3...

the current study evaluated the effects of electron beam (eb; 10, 20, 30 and 40 kgy), urea and naoh treatments on chemical composition, phenolic compounds, in vitro gas production and in situ ruminal degradability of pistachio by-product (pb). the eb decreased (p < 0.01) neutral detergent fiber (ndf), acid detergent fiber (adf) and acid detergent lignin (adl) contents of pb. treating pb by naoh...

The aim of this work was to compare use of an o-phthaldialdehyde (OPA) colorimetric assay (OPA-C), which responds to both free AA and peptides, with an OPA fluorimetric assay (OPA-F), which is insensitive to peptides, to quantify rates of ruminal protein degradation in the inhibitor in vitro system using Michaelis-Menten saturation kinetics. Four protein concentrates (expeller-extracted soybean...

Rates and extents of ruminal protein degradation for casein, solvent soybean meal (SSBM), expeller soybean meal (ESBM), and alfalfa hay were estimated from net appearance of NH3 and total amino acids in in vitro media containing 1 mM hydrazine and 30 mg/L of chloramphenicol. Protein was added at 0.13 mg of N/mL of medium, and incubations were conducted for 4 to 6 h, usually with hourly sampling...

The digestive responses and degradation of ergovaline and production of lysergic acid in the rumen of sheep offered Neotyphodium coenophialum-infected tall fescue straw at 2 ergovaline levels were investigated. Six crossbred wethers (56 +/- 3.0 kg of BW) were used in a randomized crossover design involving 2 treatments, for a total of 6 observations per treatment. The experiment consisted of tw...

Two experiments were conducted to evaluate the effects of the level of corn dry distillers grains with solubles (CDDGS) supplementation on growing performance, blood metabolites, digestion characteristics and ruminal fermentation patterns in steers grazing dormant forage. In Exp. 1, of growth performance, 120 steers (204±5 kg initial body weight [BW]) were distributed randomly into 3 groups (ea...

The potential interaction between grain (starch) and protein sources with varying ruminal degradation rates on N utilization in growing lambs was evaluated. Three grain sources with varying ruminal degradation rates, (barley greater than steam-flaked sorghum [SFSG] greater than dry-rolled sorghum [DRSG]) and three protein sources (urea greater than a 50:25:25 mixture of urea: blood meal:corn gl...

Two separate studies were conducted using a continuous culture fermenter system to determine effects of supplementing D,L-methionine and various methionine derivatives on degradation of methionine by ruminal bacteria. A basal diet containing 20% alfalfa hay, 20% corn silage and 60% grain mix (DM basis) was provided at a rate of 75 g DM/d per fermenter and served as an unsupplemented control in ...

Aspergillus oryzae fermentation extract (Amaferm) was evaluated for its ability to influence degradation of brome grass and switchgrass fiber fractions by mixed ruminal microorganisms in vitro. Addition of Amaferm at a concentration of 0.067 mg/ml, which is approximately the concentration found in the rumen ecosystem (0.06 mg/ml), increased the degradation of brome grass neutral detergent fiber...