clavulanic acid is produced industrially by fermentation of streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant dna technology, and media composition and growth condition optimization. the main objective of this study was to increase the level of clavulanic acid production from streptomyces clavuligerus (dsm 738), using uv irradiation. after...

nowadays microbial biotechnology is considered as one of the most important and profitable branches of industry. in this approach, by using the generator microorganisms, we are able to produce biotechnological products, with higher efficiency. clavulanic acid is being used in combination with commonly used beta-lactam antibiotics in order to fight against bacterial infections that are resistant...

A hybrid modeling technique is proposed to make models during the development phase of a fed-batch bioprocess. A limited amount of data is available and experimental data are therefore combined with a priori information about the mass balances and the chemical reaction network inside the cell (the metabolic network). This information highly constraints the model, and only a few degrees of freed...

Cell-free extracts prepared by sonication of Streptomyces clavuligerus cyclized 6-(L-aaminoadipyl)-L-cysteinyl-D-valine (ACV) into a penicillin-type antibiotic. The antibacterial spectrum of this antibiotic suggested it was a mixture of isopenicillin N and penicillin N indicating that both cyclization and racemase activities were present. Cyclization activity was optimal in extracts prepared fr...

The lack of reliable reference genes (RGs) in the genus Streptomyces hampers effort to obtain the precise data of transcript levels. To address this issue, we aimed to identify reliable RGs in the model organism Streptomyces coelicolor. A pool of potential RGs containing 1,471 genes was first identified by determining the intersection of genes with stable transcript levels from four time-series...

Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...

Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clavuligerus (DSM 738), using UV irradiation. After...