A protected linear polypeptide of 124 amino acid residues with the sequence of bovine pancreatic ribonuclease A was synthesized by the solid phase method. The polypeptide was removed from the solid support and pursed, and the four disulfide bonds were closed by air oxidation of the reduced form. The synthetic enzyme was fractionated by gel filtration and ion exchange chromatography, and the mat...

Local and Long-Range Electrostatic Interactions in Denatured Ribonuclease Sa: Comparing the Denatured Protein with Model Peptides (April 2002) Michael Douglas Daily Department of Biochemistry and Biophysics Texas A&M University Fellows Advisor: Dr. J. Martin Scholtz Department of Biochemistry and Biophysics The folding ol'proteins into three-dimensional, biologically active conformations demand...

In self-incompatible (SI) plants, the S locus acts to prevent growth of self-pollen and thus promotes outcrossing within the species. Interspecific crosses between SI and self-compatible (SC) species often show unilateral incompatibility that follows the SI x SC rule: SI species reject pollen from SC species, but the reciprocal crosses are usually compatible. The general validity of the SI x SC...

Endoribonuclease E (RNase E) is a regulator of global gene expression in Escherichia coli and is the best studied member of the RNase E/G ribonuclease family. Homologues are present in other bacteria but the roles of plant RNase E/G-like proteins are not known. Arabidopsis thaliana contains a single nuclear gene (At2g04270) encoding a product with the conserved catalytic domain of RNase E/G-lik...