In a recent paper, S. R. Benedict? describes a calorimetric copper method for the determination of the blood sugar which is based upon the idea that his well known copper citrate reagent should prove as superior for quantitative work on blood as it has been for qualitative tests on urine. This expectation seems to have been fulfilled to a certain extent, for by a series of paralleL blood sugar ...

In a recent paper, S. R. Benedict? describes a calorimetric copper method for the determination of the blood sugar which is based upon the idea that his well known copper citrate reagent should prove as superior for quantitative work on blood as it has been for qualitative tests on urine. This expectation seems to have been fulfilled to a certain extent, for by a series of paralleL blood sugar ...

We have adapted for glucose determination a new approach to kinetic analyses [Anal. Chem. 50, 1611 (1978)]; it is 50-fold less dependent upon some experimental variables than is a more conventional rate method. Modification of a commercially available hexokinase/glucose-6-phosphate dehydrogenase reagent system for glucose provides that the rate of production of NADH be first-order in total gluc...

The fluorescent chiral tagging reagent, 4-(3-isothiocyanatopyrrolidin-1-yl)7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole (DBD-PyNCS), was utilized for the resolution of thiol enantiomers as diastereomeric derivatives. The reagent reacts with thiol enantiomers in the presence of pyridine to produce the corresponding dithiocarbamate diastereomers under mild reaction conditions (50 °C for 40 ...

The kinetics of the hydrolysis of Woodward’s Reagent K has been studied as a function of pH in aqueous solution. Rate constants for the reagent transformation have been combined with rate data for loss of staphylococcal nuclease activity to provide the mechanism for the protein modification. The reactive species for derivatization of the enzyme is the intermediate ketoketenimine. The pH depende...

In a recent paper, S. R. Benedict? describes a calorimetric copper method for the determination of the blood sugar which is based upon the idea that his well known copper citrate reagent should prove as superior for quantitative work on blood as it has been for qualitative tests on urine. This expectation seems to have been fulfilled to a certain extent, for by a series of paralleL blood sugar ...

In a recent paper, S. R. Benedict? describes a calorimetric copper method for the determination of the blood sugar which is based upon the idea that his well known copper citrate reagent should prove as superior for quantitative work on blood as it has been for qualitative tests on urine. This expectation seems to have been fulfilled to a certain extent, for by a series of paralleL blood sugar ...

material (4 ). It appears that intermittent interferences from Hb C and S traits with some, but not all, ionexchange GHb methods continues to be an issue. In summary, some current GHb methods show clinically significant interferences with samples containing Hb C or S trait. These interferences are not necessarily consistent within method types, and with ion-exchange methods may vary over time w...

We describe a simple, noninstrumental procedure for detecting hemoglobin S in samples of blood on filter paper, with use of small volumes of reagents. The method depends on the differential solubilities of reduced sickling and nonsickling hemoglobin in phosphate solutions (1, 2) or Sickledex reagent’ and the apparent solubilizing effect of urea on reduced hemoglobin S (3). The color of paired p...

Drug immunoassays with use of reagent strips have been introduced by the Ames Co. for theophylline and phenytoin assays. Their phenobarbital assay is based on the ais (Apoenzyme Reactivation Immunoassay System) principle. Thirty microlitres of diluted serum is applied to the reagent pad and the strip is read in the Seralyzer reflectance photometer after 85 s. Fifty-three serum samples assayed b...