BACKGROUND Coeliac disease is an autoimmune disorder resulting from an interaction between diet, genome and immunity. The treatment of CoeD is lifelong adherence to a gluten free diet, which is associated with clinical and histological improvements. However, a substantive number of individuals report only partial symptom improvement despite both compliance with a strict gluten free diet and imp...

Hsp100 chaperones protect microorganisms and plants from environmental stress by cooperating with Hsp70 and its nucleotide exchange factor (NEF) and Hsp40 cochaperones to resolubilize proteins from aggregates. The Saccharomyces cerevisiae Hsp104 (Sc-Hsp104)-based disaggregation machinery also is essential for replication of amyloid-based prions. Escherichia coli ClpB can substitute for Hsp104 t...

The spread of microbial resistance around the world is one most serious threats to public health. Every year three antibiotics are released market, as each antibiotic has a limited effective lifespan after which microorganisms develop resistance. need identify new natural antimicrobial agents an important research goal at global level. use volatile plant extracts represents low risk for develop...

Mis16 and Mis18 are subunits of a protein complex required for incorporation of the histone H3 variant CenH3 (Cnp1/CENP-A) into centromeric chromatin in Schizosaccharomyces pombe and mammals. How the Mis16-Mis18 complex performs this function is unknown. Here, we report that the Mis16-Mis18 complex is required for centromere localization of Scm3(Sp), a Cnp1-binding protein related to Saccharomy...

Prickly pear (Rosa roxburghii Tratt), a shrub mainly distributed in South China, is an economically essential plant for helping the local people out of poverty. To efficiently provide sufficient nutrients to soil ecological cultivation prickly pear, we studied aerobic composting litter with three agents, including AC (Bacillus natto, Bacillus sp., Actinomycetes Saccharomyces Trichoderma Azotoba...

A cloned DNA fragment from Anabaena sp. strain PCC 7120 that complements an arginine auxotrophic mutant from the same organism was found to include an open reading frame encoding a 427-residue polypeptide that is homologous to N-acetylornithine aminotransferase from Bacillus subtilis, Escherichia coli, and Saccharomyces cerevisiae. The gene encoding N-acetylornithine aminotransferase in bacteri...

We propose a simple technique for bacterial and yeast cfu estimations from diverse samples with no prior idea of viable counts, designated as single plate-serial dilution spotting (SP-SDS) with the prime recommendation of sample anchoring (100 stocks). For pure cultures, serial dilutions were prepared from 0.1 OD (100) stock and 20 μl aliquots of six dilutions (101-106) were applied as 10-15 mi...

Cyanobacteria are able to produce extracellular substances with different biological activities and behaviors. The marine cyanobacteria Anabaena sp. strain Hi 26 and Oscillatoria subtilissima strain Bo 62 cause significant color changes in their growth media, while viscosity of the medium is influenced by Rivularia sp. strain Bo 85 and Oscillatoria limnetica strain Flo 1. Sterile-filtered media...

Objetivo: Relatar seis casos de fungemia por Saccharomyces cerevisiae, em pacientes internados Unidade Terapia Intensiva Hospital Geral São Paulo/SP/Brasil e a relação com uso probióticos. Método: levantamento retrospectivo dos dados prontuário hospitalar. Discussão: Analisando os 6 constatamos idade média 65,8 anos, predominância no sexo masculino (4/6), associação cardiopatias pneumopatias (5...