In this experiment, the action mechanism of gene Ssc1 was studied in process interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It found that could enhance pathogenicity Botrytis cinerea expression. Fusing promoter, SP CTP GFP expressing tobacco, it fusion protein be secreted into plant cells located chloroplasts. Trypan blue staini...

Plant pathogens with a broad host range are able to infect plant lineages that diverged over 100 million years ago. They exert similar and recurring constraints on the evolution of unrelated plant populations. Plants generally respond with quantitative disease resistance (QDR), a form of immunity relying on complex genetic determinants. In most cases, the molecular determinants of QDR and how t...

Oxalic acid is an important virulence factor for disease caused by the fungal necrotrophic pathogen Sclerotinia sclerotiorum, yet calcium oxalate (CaOx) crystals have not been widely reported. B. carinata stems were infected with S. sclerotiorum and observed using light microscopy. Six hours post inoculation (hpi), CaOx crystals were evident on 46% of stem sections and by 72 hpi on 100%, demons...

Resistance of benzimidazole fungicides is related to the point mutation of the β-tubulin gene in Sclerotinia sclerotiorum. The point mutation at codon 198 (GAG → GCG, E198A) occurs in more than 90% of field resistant populations in China. Traditional detection methods of benzimidazole-resistant mutants of S. sclerotiorum are time-consuming, tedious and inefficient. To establish a suitable and r...

AIMS To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. METHODS AND RESULTS The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot-grown let...

Scott, J. B., Gent, D. H., Pethybridge, S. J., Groom, T., and Hay, F. S. 2014. Crop damage from Sclerotinia crown rot and risk factors in pyrethrum. Plant Dis. 98:103-111. Sclerotinia crown rot, caused by Sclerotinia sclerotiorum and S. minor, is a prevalent disease in pyrethrum fields in Australia. Management involves fungicide applications during the rosette stage of plant development from au...

Cecile Lahiri, Emory University Katherine B. Dugan, Emory University Xianhong Xie, Albert Einstein College of Medicine Laura Reimers, Albert Einstein College of Medicine Robert D. Burk, Albert Einstein College of Medicine Kathryn Anastos, Albert Einstein College of Medicine Stewart Massad, Washington University Isam-Eldin Eltoum, University of Alabama at Birmingham Xiaonan Xue, Albert Einstein ...

Jingjing ZHANG, Yuxin LIN, Guojun DAI*, Tingting AN, Mingming SUN, Genxi ZHANG, Kaizhou XIE, Jingyu WANG College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Key Laboratory for Animal Genetics, Breeding and Reproduction and Molecular Design of Jiangsu Province, Yangzhou, P.R. China Department of Animal Science, College of Agriculture and Natural Resources, Univers...

Pseudomonas sp. DF41 is a biocontrol agent capable of suppressing Sclerotinia sclerotiorum-mediated stem rot of canola. Using transposon mutagenesis, we identified two mutants with greatly reduced antifungal (AF) activity. The first mutant had an insertion in gacS, forming part of the GacS/GacA regulatory system. The second mutation was in a gene involved in lipopeptide (LP) synthesis suggestin...

Sclerotinia sclerotiorum D-E7 was studied to determine the impact of nutritional supplements and monosaccharides on growth, oxalate accumulation, and culture pH in broth media (initial pH c. 5). Cultures with 0.1% nutritional supplement (tryptone, yeast extract, or soytone) yielded minimal growth, 2-3 mM oxalate, and a final culture pH of 4.2-4.8. In contrast, cultures with 0.1% nutritional sup...