Many steps in the replication cycle of cytomegalovirus (CMV), like cell entry, capsid assembly, and egress of newly synthesized virions, have not been completely analyzed yet. In order to facilitate these studies, we decided to construct a recombinant CMV that incorporates the green fluorescent protein (GFP) into the nucleocapsid. A comparable herpes simplex virus type 1 (HSV-1) mutant has rece...

We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally...

We introduce the SCP the sorted common prefix, and study some of its properties. Based on the internal representations used by a class of new compression schemes, we show how the SCP table can be constructed using an ( ) max κ Σ + u O number of comparisons on average, and ( ) Σ u O worst case, where u is the size of the sequence, Σ is the number of symbols, and max κ is the maximum SCP value. W...

A combination of TLC-bioautography, MALDI-TOF-MS, and LC-MS/MS methods was used to identify flavonoids with anti-α-amylase activity in extracts L. pratensis (herb), polyphillus (fruits), T. lanceolata R. Br. S. japonica (buds). After TLC-autobiography assay, substances anti-amylase were identified by MALDI-TOF-MS followed confirmation the result LC-MS/MS. Results study revealed that apigenin, l...

Abstract In 2017 the Scottish Government passed Child Poverty (Scotland) Act with commitment to significantly reduce relative child poverty rate from current prevailing level of around 25% 10% by 2030/31. response, government introduced Payment (SCP) that provides a direct transfer households at fixed per eligible – currently £25 week. this paper we explore, using micro macro modelling approach...

INTRODUCTION Sphenochoanal polyps (SCP) grow from the sphenoid sinus and occupy the nasopharynx. OBJECTIVE AND RESULTS To describe the clinical and diagnostic features of SCP, reporting two cases. Epidemiological study of the series in the literature. 8 published series were found (22 cases). CONCLUSIONS SCP is a rare lesion that is usually misdiagnosed for antrochoanal polyposis. A proper ...

Applying machine learning techniques to large datasets of in situ analyses has been proven be a powerful tool Earth Sciences. However, problems may arise when dealing with minerals such as chlorite, that exist solid solution rather than single, stoichiometric ideal. It can difficult determine whether the variations major element concentrations are due compositional difference mineral interest o...

There is increasing interest in natural flood management (NFM) and the delivery of public environmental goods. Yet implementation NFM can be ad-hoc regionally diverse. Communities often play a role thus we assess governance UK communities' position within it. We develop theoretical framework using concepts goods, social capital, collective action polycentrism use it to examine design Pickering ...

This work deals with the stochastic modelling of correlation in finance. It is well known that the correlation between financial products, financial institutions, e.g., plays an essential role in pricing and evaluation of financial derivatives. Using simply a constant or deterministic correlation may lead to correlation risk, since market observations give evidence that the correlation is hardl...

A new method for the efficient solution of free material optimization problems is introduced. The method extends the sequential convex programming (SCP) concept to a class of optimization problems with matrix variables. The basic idea of the new method is to approximate the original optimization problem by a sequence of sub-problems, in which nonlinear functions (defined in matrix variables) ar...