Utilizing the gel electrophoresis/DNA binding assay and a new technique of direct binding of radioactive DNA to protein blots, we have investigated putative factors selective for the cAMP-responsive element (CRE) of the lactate dehydrogenase A subunit promoter in rat ovary nuclear extracts. Analysis of linker-scanning mutants of lactate dehydrogenase A subunit promoter fragments by DNA binding ...

The heritability of cancers is usually affected by complex interactions between carcinogens and the host's genome. New aspects of the genetics of cancer pathogenesis, such as DNA methylation, and micro RNAs are increasingly recognized as important events. Chemotherapeutic drugs interfere with cell division in different ways, e.g. with the duplication of DNA or the separation of newly formed chr...

The binding mechanism of a new class of lipid-competitive, ATP non-competitive, p110α isoform-selective PI3K (phosphoinositide 3-kinase) inhibitors has been elucidated. Using the novel technique of isoform reciprocal mutagenesis of non-conserved amino acids in the p110α and p110β isoforms, we have identified three unique binding mechanisms for the p110α-selective inhibitors PIK-75, A-66S and J-...

background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...

A new carbohydrate receptor possesses a C3-symmetric polar cavity capable of encapsulating disaccharides; binding to beta-maltosyl is preferred, complementing previous systems which have favoured "all-equatorial" substrates.