In this study, neural cell sorting of high throughput and high purity is made using two-step separation by combining soft inertial microfluidics and pinched flow fractionation. Both finite element analysis tool and equivalent electric circuits modeling were used to design the microfluidic devices. Separation between neurons and glia cells from rat’s spine cord was demonstrated. To minimize the ...

Individual tissues of complex eukaryotic organisms have specific gene expression programs that control their functions. Therefore, tissue-specific molecular information is required to increase our understanding of tissue-specific processes. Established methods in plants to obtain specific tissues or cell types from their organ or tissue context typically require the enzymatic degradation of cel...

We explore the passive optical sorting of plasmon nanoparticles and investigate the optimal wavelength and optimal beam shape of incident field. The condition for optimal wavelength is found by maximising the nanoparticle separation whilst minimising the temperature increase in the system. We then use the force optical eigenmode (FOEi) method to find the beam shape of incident electromagnetic f...

Cell sorting is an important technology that is widely used for medical diagnosis in hospitals and cell engineering research. Among cell sorting technology, dielectrophoresis (DEP) is one of the most promising approaches for manipulating and separating biological particles because this phenomena requires no labeling procedure with a fluorescent dye or magnetic beads. In this study, we developed...

The ability to pre-select offspring sex via separation of X- and Y-bearing sperm would have profound ramifications for the animal husbandry industry. No fully satisfactory method is as yet available for any species, although flow sorting is commercially viable for cattle. The discovery of antigens that distinguish X- and Y-bearing sperm, i.e. offspring sex-specific antigens (OSSAs), would allow...

The objective of this study was to assess the utility of the magnetic-activated cell sorting (MACS) technique used for improving characteristics and quality of insemination doses by the elimination ofapoptotic rabbit spermatozoa from a heterospermic pool (Experiment 1) as well as from the ejaculates of individual bucks (Experiment 2). Superparamagnetic microbeads conjugated with annexin V elimi...

Recent studies show that visual information contained in visual speech can be helpful for the performance enhancement of audio-only blind source separation (BSS) algorithms. Such information is exploited through the statistical characterisation of the coherence between the audio and visual speech using, e.g. a Gaussian mixture model (GMM). In this paper, we present two new contributions. An ada...

The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and...

We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed in vivo with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is obse...

Microfluidic and lab-on-a-chip devices have gained widespread attendance in separation sciences since they are characterized by advantageous properties like a gain in throughput, time, cost, performance and analyte amount. Beyond these advantages, microfluidic devices open access to completely new separation or particle sorting phenomena far from thermal equilibrium based on Brownian motion and...