Xylan-type polysaccharides were isolated from the leaves of Argania spinosa (L.) Skeels collected in the Tindouf area (southwestern Algeria). Xylan fractions were obtained by sequential alkaline extractions and purified on Sepharose CL-4B. The xylan structure was investigated by enzymatic hydrolysis with an endo-β(1→4)-xylanase followed by chromatography of the resulting fragments on Biogel P2,...

Cibacron Blue 3G-A inhibited monkey liver serine hydroxymethyltransferase competitively with respect to tetrahydrofolate and non-competitively with respect to L-serine. NADH, a positive heterotropic effector, failed to protect the enzymes against inhibition by the dye and was unable to desorb the enzyme from Blue Sepharose CL-6B gel matrix. The binding of the dye to the free enzyme was confirme...

for the samples incubated with and without Glc6P) makes it tedious and slow. Moreover the response to different concentrations of the material retarded on the Sepharose CL-6B columns was very non-linear. However, at present this approach seems the only certain way forward towards identifying the site of action of hexose phosphates. Although the identity of the protein remains unknown, we can at...

Described is a two-chromatographic-step preparative-scale technique for the purification of human prolactin from a frozen pituitary homogenate. The method utilizes hydrophobic interaction chromatography on the mildly hydrophobic adsorbent phenyl-Sepharose CL-4B and anion-exchange chromatography on DEAE-cellulose in the presence of acetonitrile. Human prolactin was solubilized at pH10.0 after a ...

The IgM in three patients with paraproteinemia and peripheral neuropathy was shown to bind to human myelin-associated glycoprotein (MAG) that had been purified to homogeneity by gel filtration on Sepharose CL-6B. The antigenic determinant reacting with the IgM from all three patients was in the carbohydrate part of the MAG molecule. In addition, the IgM from the same three patients bound to a s...

Hepatitis B surface antigen immunopurification using a plant-derived specific antibody coupled to Sepharose CL-4B at different ligand densities Rodolfo Valdés, Yenisley Medina, William Ferro, José A. Montero, Tatiana Alvarez, Déborah Geada, Alberto Leyva, Leonardo Gómez, Sigifredo Padilla, Biunayki Reyes, Leonardo Pacín, Alejandro Figueroa, Andrés Tamayo, Lorely Milá, Yurisleydi Aldama and Marí...

In order to improve the specificity and sensitivity of the techniques for the human anisakidosis diagnosis, a method of affinity chromatography for the purification of species-specific antigens from Anisakis simplex third-stage larvae (L3) has been developed. New Zealand rabbits were immunized with A. simplex or Ascaris suum antigens or inoculated with Toxocara canis embryonated eggs. The IgG s...

Ribosome-inactivating proteins (RIPs) are plant toxins that irreversibly damage ribosomes and other substrates, thus causing cell death. RIPs classified in type 1 RIPs, single-chain enzymatic proteins, 2 consisting of active A chains, similar to linked lectin B which enable the rapid internalization toxin into cell. For this reason, many very cytotoxic, ricin, volkensin stenodactylin being most...

acetone fractionated peroxidase from crude extract of brassica oleracea leaves (cabbage) was purified in three steps on chromatographic columns, using sp-sepharose, deae-sepharose and con a-sepharose. the specific activity of purified main isoenzyme (boc-pod) is 1887 u/mg protein with rz: 3.1, which is 172 times more than the rz of crude extract with 4.3% recovery. the molecular weight of boc-p...