Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, the former often do not work effectively and the latter may be prohibitively expensive if a large number of fractionations are re...

Afforestation is a prevalent practice carried out for soil recovery and carbon sequestration. Improved understanding of the effects of afforestation on soil organic carbon (SOC) content and dynamics is necessary to identify the particular processes of soil organic matter (SOM) formation and/or decomposition that result from afforestation. To elucidate these mechanisms, we have used a sequential...

Pollution of water bodies by phosphorus in runoff from soil amended with animal manures is one of the greatest threats to water quality in developed countries. The environmental fate of manure phosphorus is determined in part by its chemical composition, yet extraction procedures to assess this are poorly developed and provide no structural information. We used solution 31P NMR spectroscopy to ...

Abstract The transformation of technogenic Cu and Zn compounds in technogenically transformed soils (Spolic Technosols) with high very concentrations metals formed at the site a natural tailings pond floodplain Seversky Donets River, main tributary Don River (Rostov oblast, Russia) has been studied. Technosols are compared to an unpolluted meadow-chernozemic soil (Fluvisol) outside impact zone....

0

Wheat bran is a major by-product of cereal production that still has limited use for advanced nutritional and material applications. A sequential process using subcritical water, membrane filtration and selective enzymatic treatments has been designed for the combined fractionation of functional high molar mass hemicelluloses (over 10 g mol) and oligosaccharides from wheat bran. This process no...