Abstract INTRODUCTION Given the limited drug penetration across blood-brain barrier (BBB), therapeutic potential of new and existing therapies has not been fully exploited for benefit glioblastoma (GBM) patients. METHODS Here we employed a novel delivery technology based on low-intensity pulsed ultrasound combined with intravenous microbubbles (LIPU/MB) that temporarily opens BBB to deliver lip...

Arylamine N-acetyltransferase (NAT) genes in humans and in rodents encode polymorphic drug metabolizing enzymes. Human NAT1 (and the murine equivalent mouse Nat2) is found early in embryonic development and is likely to have an endogenous role. We report the detailed expression of the murine gene (Nat2) and encoded protein in mouse embryos, using a transgenic mouse model bearing a lacZ transgen...

background: the study of specific genes expressed in the testis is important to understanding testis development and function. spermatogenesis is an attractive model for the study of gene expression during germ cell differentiation. spermatogenesis associated-19 (spata-19) is a recently-identified important spermatogenesis-related gene specifically expressed in testis. its protein product is in...

Abstract The zinc finger protein, Helios, is a member of the Ikaros transcription factor family and expressed in CD4 +T cells, CD8 cells some NK both mice humans. While it has been found to have role homeostasis suppressive function +Foxp3 regulatory (Treg), its not well characterized. We demonstrated that ~10–40% from normal donors express Helios. Expression Helios +appears be downregulated wi...

UNLABELLED Human La protein is known to be an essential host factor for translation and replication of hepatitis C virus (HCV) RNA. Previously, we have demonstrated that residues responsible for interaction of human La protein with the HCV internal ribosomal entry site (IRES) around the initiator AUG within stem-loop IV form a β-turn in the RNA recognition motif (RRM) structure. In this study, ...

The collagen binding chaperone HSP47 interacts with procollagen in the endoplasmic reticulum and plays a crucial role in the biosynthesis of collagen. We recently demonstrated that typical collagen model peptides, (Pro-Pro-Gly)(n), possess sufficient structural information for interaction with HSP47 (Koide, T., Asada, S., and Nagata, K. (1999) J. Biol. Chem. 274, 34523-34526). Here we show that...

Hermansky-Pudlak syndrome (HPS) is a disorder of organelle biogenesis affecting 3 related organelles-melanosomes, platelet dense bodies, and lysosomes. Four genes causing HPS in humans (HPS1-HPS4) are known, and at least 15 nonallelic mutations cause HPS in the mouse. Where their functions are known, the HPS-associated proteins are involved in some aspect of intracellular vesicular trafficking,...

Abstract Background Somatic stem cell transplantation has been performed for cartilage injury, but the reparative mechanisms are still conflicting. The chondrogenic potential of cells thought as promising features therapy; however, correlation between their chondrogenesis in vitro and vivo remains undefined. purpose this study was to investigate intrinsic condition depends on types explore an i...

The DDB2 gene, which is mutated in xeroderma pigmentosum group E, enhances global genomic repair of cyclobutane pyrimidine dimers and suppresses UV-induced mutagenesis. Because DDB2 transcription increases after DNA damage in a p53-dependent manner, we searched for and found a region in the human DDB2 gene that binds and responds transcriptionally to p53. The corresponding region in the mouse D...

The study of comorbidity can provide new insights into the pathogenesis disease and has important economic significance in clinical evaluation treatment difficulty, medical expenses, length stay, prognosis disease. In this paper, we propose a association prediction model DapBCH, which constructs cross-species biological network applies heterogeneous graph embedding to predict association. First...