The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...

in order to investigate the in vitro micropropagation hibiscus rosa-sinensis three tests in a completely randomized design with 10 replications were performed in tissue culture laboratory shahid chamran university in 2014.the first experiment showed the perfect media.second tests best consentration bap for multiplication and third tests showed the best type and concentration rooting hormone. co...

To enhance the genetic resource base of Echinacea purpurea (L.) Moench, a tetraploid was developed by treating seeds with different concentrations of colchicine. For further multiplication, in vitro micropropagation through adventitious shoot induction and proliferation was achieved. Shoot buds were induced directly on adaxial surface of mature leaf tissues growing on MS medium supplemented wit...

the influence of different initial inoculum densities of the root-knot nematode meloidogyne javanica on the growth of sage (salvia officinalis), root-knot development and nematode multiplication was investigated 90 days after inoculation under greenhouse conditions. with the increase in the nematode initial inoculum densities, shoot fresh/dry weight, shoot/root length and root fresh/dry weight ...

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...

The conventional methods of propagation of bamboos sexual as well as vegetative have lots of problems that restrict their multiplication in large numbers. Bamboo is now a commercial crop and hence there is shortage of availability of planting stock material. To overcome this tissue culture offers rapid and reliable method to produce plants in large number. Tissue culture protocol has been devel...

The influence of different initial inoculum densities of the root-knot nematode Meloidogyne javanica on the growth of sage (Salvia officinalis), root-knot development and nematode multiplication was investigated 90 days after inoculation under greenhouse conditions. With the increase in the nematode initial inoculum densities, shoot fresh/dry weight, shoot/root length and root fresh/dry weight ...

Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution diseases. Micropropagation is the only practical means achieving rapid, large-scale production disease-free quality planting material. Experiments on shoot tip culture initiation were laid out in completely randomized design with 2x3x3 4x5x3 factorial treatment arrangements respectivel...