in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the...

Plants were regenerated from encapsulated shoot tips of pointed gourd. Shoot tips isolated from multiple shoot cultures of AM-8 and AM-15 cultivars of pointed gourd were encapsulated in sodium alginate beads. For germination and shoot proliferation, encapsulated shoot tips (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA. Use...

A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures

Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from ...

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy INDIRECT SHOOT ORGANOGENESIS AND SELECTION OF SOMACLONAL VARIATION IN Dieffenbachia 11 By Xiuli Shen May 2007 Chair: Michael E Kane Cochair: Jianjun Chen Major: Horticultural Science A series of experiments were conducted to investigate ...

Vitex trifolia is a shrub species with popular use as a medicinal plant, for which leaves, roots and flowers have been reported to heal different distresses. The increasing exploitation of these plants has endangered its conservation, and has importantly justified the use of biotechnological tools for their propagation. Our aim was to present an efficient protocol for plant regeneration through...

Plants have a profound capacity to regenerate organs from differentiated somatic tissues, based on which propagating plants in vitro was made possible. Beside its use in biotechnology, in vitro shoot regeneration is also an important system to study de novo organogenesis. Phytohormones and transcription factor WUSCHEL (WUS) play critical roles in this process but whether and how epigenetic modi...

Medium composition for the callus induction, shoot multiplication in four Indian medicinal plants, Boerhaavia diffusa, Baliospermum axillare, Abutilon indicum and Nymphoides cristatum were standardized using Murashige and Skoog`s plant tissue culture medium as the basal medium. The protocol thus standardized was further evaluated replacing sucrose and vitamins with the fruit pulp of Borassus fl...