Boronic acid transition state inhibitors (BATSIs) with R1 side chains of cefotaxime and ceftazidime were assayed against SHV-1, SHV-2, SHV-5, D104K, and D104K G238S beta-lactamases. The D104K variant was the most susceptible to inhibition by the ceftazidime BATSI (Ki, 730+/-80 nM), while the D104K G238S variant was the most susceptible to the cefotaxime BATSI (Ki, 1.1+/-0.2 microM).

Plasmid-encoded resistance to broad-spectrum cephalosporins and aztreonam is becoming a widespread phenomenon in clinical medicine. These antibiotics are inactivated by an array of different extended-spectrum beta-lactamases (ESBLs) which have evolved by point mutations of parental TEM or SHV beta-lactamases. In a previous study conducted during 1994-1995, SHV-2, SHV-2a and SHV-5 beta-lactamase...

background and objective: nowadays, the presence of extended-spectrum β-lactamases (esbls) producing strains in serratia genus causes the emergence of resistance to many antibiotics. so, the lack of proper diagnosis of esbls strains can lead to failure in the treatment. the objective of the present study was to investigate esbls production in serratia strains isolated from the clinical blood sa...

Background: The prevalence of ESBLs-producing Escherichia coli has recently increased worldwide due to the expression ESBL genes which had led high rate multidrug resistance antibiotics. Aim: To determine antimicrobial susceptibility patterns E. isolates and evaluating carriage these at phenotypic genotypic levels. Methods: One hundred two clinical were recovered from UTI suspects analyzed for ...

BACKGROUND/PURPOSE The emergence of multiple drug resistance in Enterobacteriaceae is of particular concern. The aim of this study was to evaluate the antimicrobial susceptibility and screen for the ampC gene in three members of the Enterobacteriaceae family (Enterobacter cloacae, Citrobacter freundii, and Serratia marcescens) found at Taichung Veterans General Hospital during the past 5 years ...

Genes encoding the CMY-2, CTX-M-14, and SHV-12 beta-lactamases were detected in three of five Escherichia coli isolates from fecal samples from healthy chickens which showed resistance or diminished susceptibility to extended-spectrum cephalosporins. A -42 mutation at the promoter region of the ampC gene was detected in the other two isolates.

10.1128/JCM.41.3.1161-1166.2003. 2003, 41(3):1161. DOI: J. Clin. Microbiol. Alex van Belkum and Henri Verbrugh Nicole Lemmens-den Toom, Marga van Santen-Verheuvel, Paul Gruteke, Wil Goessens, Jan van Gils, Paul Peerbooms, Causing a Nosocomial Outbreak Klebsiella pneumoniae Efflux Pump of -Lactamase SHV-5 Gene, and a Multidrug β Integrons, the Extended-Spectrum Patterns of Resistance Associated ...

OBJECTIVES The aim of this study is to evaluate the prevalence and diversity of extended-spectrum beta-lactamases (ESBLs) in Enterobacter cloacae clinical isolates collected from Algerian hospitals and to verify the association with qnr genes. METHODS MICs were determined by Etest for isolates giving positive double-disc synergy tests, and all isolates were screened by PCR and sequenced, resp...

We investigated the diversity of the chromosomal class A beta-lactamase gene in Klebsiella pneumoniae in order to study the evolution of the gene. A 789-bp portion was sequenced in a panel of 28 strains, representative of three phylogenetic groups, KpI, KpII, and KpIII, recently identified in K. pneumoniae and of different chromosomal beta-lactamase variants previously identified. Three groups ...

La Ciudad de México (CDMX) enfrenta una situación vulnerabilidad sociohidrológica (SHV) debido a la creciente escasez agua. Este estudio evalúa SHV Alcaldía Cuajimalpa con base en construcción social del espacio. El estrés hídrico y capacidad adaptativa son elementos clave para comprender las condiciones materiales, ambientales sociales un territorio. Se propone metodología mixta como eje acció...