The Rockefeller University Press $30.00 J. Gen. Physiol. 2016 Vol. 148 No. 2 79–88 www.jgp.org/cgi/doi/10.1085/jgp.201611649 79 It is more than 30 years since the advent of the gigaohm seal patch clamp technique, which enabled routine recordings of currents generated by the activity of a single ion channel molecule in its native environment. The muscle nicotinic acetylcholine (ACh) receptor (nA...

Cristian Ionescu-Zanetti et al. have developed a technique for electrophysiological recording of whole mammalian cells on a microf luidic chip. The technique permits multiplexed recording of ion channel voltages, while leaving the cells visible for microscopic inspection and manipulation. The device records through many junctions along the microf luidic channel while a microscope observes from ...

Ion transports from one aqueous phase (W1) to another (W2) across bilayer lipid membranes (BLM) containing KAT1 potassium channel were investigated by recording current fluctuations. KAT1 channel is a voltage-gated K(+) channel from Arabidopsis thaliana and has been suggested to have a key role in stomatal opening by osmoregulation in guard cells. Although KAT1 channel is a K(+)-specific tran...

Ion transport from one aqueous (W1) to another (W2) across bilayer lipid membranes (BLM) containing gramicidin A (GA) was investigated by recording current fluctuations, when various alkali metal chlorides and potassium salts were used as supporting electrolytes. The magnitude of the single-channel current at a given membrane potential depended on not only the cationic species, but also on the ...

-Blind source separation is an advanced statistical tool that has found widespread use in many signal processing applications. However, the crux topic based on one channel audio source separation has not fully developed to enable its way to laboratory implementation. The main idea approach to single channel blind source separation is based on exploiting the inherent time structure of sources kn...

Cell-attached and inside-out excised-patch recording techniques were used to search for mechanosensitive ion channels in neonatal and adult rat atrial myocytes. A channel activated by negative pressure applied to the patch, with a single-channel conductance of 52 pS in symmetric potassium solutions, was frequently observed. This channel has been identified as the atrial ATP-sensitive potassium ...

We used the patch clamp technique to study the nature of the late sodium current in guinea pig ventricular myocytes. In a cell attached mode of single channel recording at room temperature (22-24 degrees C) two kinds of late (100 msec or more after beginning of the depolarizing pulse) sodium channel activities were recognized. One is isolated brief openings appearing once for about 120 depolari...

The gating mechanism of a single ion channel is usually modelled by a nite state space continuous time Markov chain. The patch clamp technique enables the experimenter to record the current owing across a single ion channel. In practice, the current is corrupted by noise and low-pass ltering, and is sampled with a typically very short sampling interval. We present a method for performing Bayesi...

Expressed in Xenopus oocytes, KvLQT1 channel subunits yield a small, rapidly activating, voltage- dependent potassium conductance. When coexpressed with the minK gene product, a slowly activating and much larger potassium current results. Using fluctuation analysis and single-channel recordings, we have studied the currents formed by human KvLQT1 subunits alone and in conjunction with human or ...