A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtrat...

The mobility of fluorescein and bromide used as tracers in packed soil columns was investigated. Five different soils were used in two application methods: soil surface application and soil incorporation, both of which simulate accepted methods of soil application of termiticides to prevent structural infestation. The breakthrough of bromide and fluorescein in column eluates were measured. The ...

Sharp, R. E and Da vies, W. J. 1985. Root growth and water uptake by maize plants in drying soil.— J. exp. Bot. 36: 1441-1456. The influence of soil drying on maize (Zea mays L.) root distribution and use of soil water was examined using plants growing in the greenhouse in soil columns. The roots of plants which were watered well throughout the 18 d experimental period penetrated the soil profi...

Soil fumigants are becoming an important source of volatile organic compounds (VOCs) in air, especially in some agricultural areas. In this study, we used thiourea to construct a reactive surface barrier (RSB) at the soil surface for reducing 1,3-dichloropropene (1,3-D) volatilization. The agrochemical thiourea could rapidly transform volatile 1,3-D to nonvolatile products via an SN2 nucleophil...

The purpose of this work is to assess the effectiveness of two grass covers (buffer zone and grass-covered inter-row), to reduce pesticide leaching, and subsequently to preserve groundwater quality. Lower amounts of pesticides leached through grass-cover soil columns (2.7-24.3% of the initial amount) than the bare soil columns (8.0-55.1%), in correspondence with their sorption coefficients. Diu...

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring m...

UNLABELLED This study demonstrates the prevalence, phylogenetic diversity, and physiology of nitrate-reducing microorganisms capable of utilizing reduced humic acids (HA) as electron donors in agricultural soils. Most probable number (MPN) enumeration of agricultural soils revealed large populations (10(4) to 10(6) cells g(-1) soil) of microorganisms capable of reducing nitrate while oxidizing ...

Recent large-scale beetle infestations have caused extensive mortality to conifer forests resulting in alterations to dissolved organic carbon (DOC) cycling, which in turn can impact metal mobility through complexation. This study analyzed soil-water samples beneath impacted trees in concert with laboratory flow-through soil column experiments to explore possible impacts of the bark beetle infe...