Assessments of bacterial community diversity and dynamics are fundamental for the understanding of microbial ecology as well as biotechnological applications. We show that the choice of PCR primers has great impact on the results of analyses of diversity and dynamics using gene libraries and DNA fingerprinting. Two universal primer pairs targeting the 16S rRNA gene, 27F&1492R and 63F&M1387R, we...

A nucleoside analogue, 1 -(2’-deoxy-~-~-ribofuranosyl)-3-nitropyrrole (4) was designed to function as a universal replacement for any of the natural nucleosides in DNA sequences. Compound 4 was synthesized by the reaction of 3-nitropyrrole with sodium hydride and l-chloro-2-deoxy-3,5-di-O-toluoyl-~-e~opentofuranose, and the structure was confirmed by X-ray diffraction. Nucleoside 4 was transfor...

in present study, a sybr green based real time pcr assay was developed for specific detection and quantification of bacillus subtilis in dough used for bread making. new primer pairs were designed to amplify a 212 base pair fragment of the apre gene. specificity of these primer pairs was confirmed with conventional and real time pcr methods. standard curves constructed using the threshold cycle...

The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as 'iap', 'hly' and 'prf' was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against different regions of 'iap' produced 371 and 660 bp PCR products, respectively. The primer pair targe...

Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences-mini-barcodes-for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species com...

Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry (Morus spp.) genotypes, including M. mongolica, M. bombycis, M. alba, M. atropurpurea and M. multicaulis. Subsequently, the polymerase chain reaction (PCR) products were digested by seven restriction enzymes and the trnD-trnT fragment for sequence alig...

To serve as an initial step in developing an ideal genetic marker map for the house musk shrew, Suncus murinus, 318 comparative anchor tagged sequence (CATS) primer pairs were assessed for polymorphism ascertainment and linkage mapping. Of the 112 (35.2%) CATS primer pairs that were successfully amplified by PCR in the shrew, 18 (16.1%) showed polymorphism between two mutant strains, BAN-kc, oe...

to evaluate allelic diversity at loci encoding low molecular weight glutenin subunits (lmw-gs) among 62 genotypes of bread wheat, nine specific primer pairs for glu-a3, glu-b3, glu-d3 loci were employed. pcr products were separated on 2% agarose gels. two pairs of specific primers, at amplified glu-a3 locus, 8 alleles were detected the c allele of which was the most abundant with a frequency of...

UNLABELLED Since bacteria remain in the dentin following caries removal, restorative materials with antibacterial properties are desirable to help maintaining the residual microorganisms inactive. The adhesive system Clearfil Protect Bond (PB) contains the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) in its primer, which has shown antimicrobial activity. However, its...

Inorganic gunshot residues (GSR) can arise from each component of the fired cartridge and GSR deposits inside the barrel from previous shootings. This work focused on the analysis of primer mixtures that yield GSR-unique particles. Primer mixtures and gunshot residues from 25 kinds of cartridges were recovered and subjected to scanning electron microscopy/ energy dispersive X-ray analysis to ob...