Approximately 2 X 10 ton biomass is produced on land and in water annually (l). Cellulose is the principal structural material in the cell wall of all plants (2). In addition, cellulose can be found in algae, bacteria, and animals (tunicates). Assuming that the average source contains 50% cellulose (Table 1) (35), then approximately 1 X 10 ton cellulose is formed each year. This makes cellulose...

INTRODUCTION Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. A variety of detection methods have been used, among them viscometry, lowangle laser light scattering (LALLS), refractive index, ultraviolet/visible absorbance, fluorescence, and precision differential refractometry coupled with ultraviolet detection.This a...

Size exclusion chromatography (SEC) is a widely used tool in bioseparations. Because its separation mechanism is based on the permeability of macromolecules rather than any type of binding, the feed volume to bed volume ratio is usually quite small. Thus, large columns are typically used in preparativeand large scale separations. In this work, a general rate model considering various mass trans...

We consider two presentations regarding the size exclusion chromatography of highly asymmetric solutes: one is embodied in the statistical model of Giddings et al.;I the second is described by the concept of "end-on insertion" proposed by Tanford* and expanded upon by Meredith and N a t h a n ~ . ~ Our evaluation is based on measurements of Kfor the rigid rodlike and spherical solutes schizophy...

A major problem in the measurement of serum bile acids is their quantitative extraction from the high molecular protein matrix. In our hands, the standard techniques of adsorption and reversed-phase chromatography have yielded incomplete recovery for different bile acids (33-93%) and poor reproducibility. In contrast, with the novel extraction procedure of size exclusion chromatography, recover...

BACKGROUND Knowledge about the presence of intact cardiac troponin T (cTnT) and/or its immunoreactive fragments is of great value for the interpretation of cTnT clearance from the circulation. Until now there has been a lot of controversy about cTnT fragmentation. To provide an answer to this controversy, we investigated fragmentation of cTnT with size-exclusion chromatography (SEC), and confir...

Branched polymers are among the most important polymers, ranging from polyolefins to polysaccharides. Branching plays a key role in the chain dynamics. It is thus very important for application properties such as mechanical and adhesive properties and digestibility. It also plays a key role in viscous properties, and thus in the mechanism of the separation of these polymers in size-exclusion ch...

INTRODUCTION Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. A variety of detection methods have been used, among them viscometry, lowangle laser light scattering (LALLS), refractive index, ultraviolet/visible absorbance, fluorescence, and precision differential refractometry coupled with ultraviolet detection.This a...

The cystic fibrosis transmembrane conductance regulator protein (CFTR) is a chloride channel highly expressed in the gills of Salmo salar, with a role in osmoregulation. It shares 60% identity with the human CFTR channel, mutations to which can cause the common genetic disorder cystic fibrosis CF. The expression and localisation of salmon CFTR have been investigated, but the isolated protein ha...

terials, operated in a pressure-driven mode, plotted as a function of inverse temperature. This Rgure is intended to give some appreciation of the Suxes that are attainable; however, they are not normalized to a given partial pressure gradient or thickness of membrane, and thus the Suxes are not directly comparable. Taking a value of between 10 and 100 L m 2 min 1 as the level of oxygen Sux nee...