In the study of the regulation of incubation, broodiness and laying performance in pigeons (Columba liva), a cDNA library, which was enriched with full-length brooding-related genes, was constructed by SMART LD-PCR techniques using the pituitary glands of incubating White King pigeons. The titers of optimal primary libraries were 1.54x10(6) pfu/mL and 1.80x10(6) pfu/mL and the titers of amplifi...

interferon regulatory factor 7 (irf7) gene was cloned from a subtractive cdna library constructed with mrnas obtained from rainbow trout (oncorhynchus mykiss) macrophage cell line (rts-11). using expressed sequence tag clones of submitted irf7 amino acid sequences, specific primers were designed. results showed that irf7 cdna contains an orf of 1251 nucleotides that translates into a 416 residu...

Tetrodotoxin (TTX)-binding proteins are present in toxic TTX-bearing animals, such as pufferfish and gastropods. These may prevent autotoxicity. However, TTX-binding also found the nontoxic marine shore crab, Hemigrapsus sanguineus. Here, we isolated protein, HSTBP (Hemigrapsus sanguineus protein), from hemolymph of H. elucidated its primary structure using cDNA cloning. HSTBP, a 400 kDa acidic...

The oocyte is a key regulator of ovarian folliculogenesis and early embryonic development. However, the composition of the oocyte transcriptome and identities and functions of key oocyte-specific genes involved in the above processes are relatively unknown. Using a PCR-based cDNA amplification method (SMART technology), we constructed a bovine oocyte cDNA library. Analysis of 230 expressed sequ...

There is increasing interest in interfacing dissociated neuronal cultures with planar multielectrode arrays (MEAs) for the study of the dynamics of neuronal networks. Here we report on the successful use of three-dimensional tip electrode arrays (3D MEAs), originally developed for use with brain slices, for recording and stimulation of cultured neurons. We observed that many neurons grew direct...

extended abstract 1. introduction today the irregular growth of the population and its illogical distribution across natural and cultural environments of different communities has necessitated sustainable development in response to the unstable conditions of cities. mashhad is a city with fast growth and development but with no clear plan, especially after land reform program. in this sense, th...

in this research, the buckling and bending behaviour of smart nanocomposite plate reinforced by single- walled carbon nanotubes (swcnts) under electro-magneto-mechanical loadings is studied. the extended mixture rule approach is used to determine the elastic properties of nanocomposite plate. equilibrium equations of smart nanocomposite plate are derived using the hamilton’s principle based on ...

It seems that virtually every technical paper which describes a new technology to "make the world a better place", includes the term "smart" to refer to some aspect of this new technology. The technical literature is filled with smart capacitors, smart resistors, smart rectifiers, smart op-amps, smart memory, smart circuits, smart cards, smart systems, smart networks, smart partners, smart opin...