Background: Interleukie-8 is a proinflammatory cytokine and a potent chemotactic and activation factor for neutrophils. It has been reported to be expressed in high levels by various cell types after immune stimulation and mediates neutrophil recruitment in host. Objectives: The aim of this study was to analysis of the relationship between single nucleotide polymorphisms (SNPs) at position -18...

Drug resistance in Plasmodium falciparum parasites is associated with the single nucleotide polymorphism (SNP) exo-E415G, which considered as a marker to reduce susceptibility artimisinin and leads treatment failure. This study aims establish procedure based on tetra-primer ARMS PCR technique detect SNP exo-E415G malaria patients who experienced failure artemisinin. DNA sample from P. 3D7 isola...

Daphnia magna belongs to the Cladocera and plays an important role in water ecosystem. With intensification of pollution, wild population D. has declined rapidly recent years, insufficient molecular markers have limited effective research conservation this species. In research, 30 novel single nucleotide polymorphism (SNP) were developed a cultivar 12 using restriction site-associated DNA seque...

The dojo loach Misgurnus anguillicaudatus is an endemic freshwater species in Asia. effective conservation and molecular-aided selection of M. were limited without sufficient molecular markers. In this study, 112 novel single nucleotide polymorphism (SNP) markers screened based on 2b-RAD sequencing database, 56 SNPs them developed characterized by genotyping 40 individuals using SNaPshot method...

Single-nucleotide polymorphism (SNP) markers have great potential to identify individuals, family relations, biogeographical ancestry, and phenotypic traits. In many forensic situations, DNA mixtures of a victim an unknown suspect exist. Extracting SNP profiles from suspect’s samples can be used assist investigation or gather intelligence. Computational tools determine inclusion/exclusion known...

AIM To analyze the distribution of the single nucleotide polymorphism (SNP) C677T in the methylenetetrahydrofolate reductase (MTHFR) gene in 355 infertile Chinese patients with idiopathic azoospermia or severe oligozoospermia and 252 fertile Chinese men as controls to explore the possible association of the SNP and male infertility. METHODS Using the polymerase chain reaction (PCR)-restrictio...

As the host’s first line of defense against pathogens, Toll-like receptors (TLRs), such as TLR3, are genes encoding transmembrane same name. Depending on their expression, TLRs cause a pro- or anti-inflammatory response. The purpose article was to determine whether there is an association between receptor 3 (TLR3) rs3775291 Single Nucleotide Polymorphism—SNP and susceptibility infections. This ...

Previous genetic studies of orangutans (Pongo spp.) have relied mainly upon mitochondrial DNA or microsatellite short tandem repeats (STR) for genomic genotyping analysis. Scientists yet to take advantage the closeness great apes humans analysis by using advanced techniques available human genotyping. To genotype at Tanjung Puting National Park, we developed a novel combination methyl-based mag...

Leptin (LEP), the expression product of the obese gene produced primarily in the adipose tissue, is related to feed intake, growth and lipid metabolism. The aim of this study was to study the possible association between polymorphism of the LEP gene with growth and carcass traits among three Iranian sheep breeds of the Shal, Zandi and Zel varieties. A total of 180 purebred animals of the Shal, ...