A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temper...

Abstract In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These were used to extract residual proteins in non-aged and thermal aged samples. The protein efficiencies different extracting quantitatively determined by bicinchoninic acid (BCA) method, then processed multivariate analysis variance (MANOVA). impact pr...

A protease produced by Vibrio mimicus was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on Sephacryl S-100 and Mono Q Monobeads. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) of the final preparation of the enzyme revealed the homogeneity of the purified enzyme. Conventional PAGE showed that the purified protease m...

Copper-zinc superoxide dismutase from Ascaris suum (Nematoda) was purified in a new, more efficient, and faster manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability,...

The murine Ss protein has been isolated and purified. Using specific antisera, the radiolabeled protein has a mol wt of 120,000 in sodium dodecyl sulfate polyacrylamide gels. It is composed of two basic subunits of 23,000 and 14,000 daltons. The smaller molecular weight subunit contains a single disulfide bridge, is devoid of carbohydrate, and may represent the murine equivalent of beta2-microg...

The similarity of yeast microtubular protein to the tubulins purified from other eukaryotic organisms has been open to question. This work involves the identification of two yeast proteins that resetile a ands tubulin prepared from other eukaryots in their ability to copolymerite with purified rat brain tubulin and co-migrate with cL and B brain tubulin on a sodium dodecyl sulfate polyacrylamid...

Ureaplasma urealyticum is an organism considered susceptible to tetracycline. Ten tetracycline-resistant (Tcr) clinical isolates and Tcr serotype 9 were examined. All contained DNA sequences homologous to the streptococcal determinant tetM. They differed from each other on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Southern blots, and immunoblots and appeared to represent differ...

this study was conducted to investigate the effect of physical and chemical processing on crude protein fractions of two varieties of canola (okapi and modena) and safflower (62 and zarfan) seed using cornel net carbohydrate and protein system and on protein subunits using sodium dodecyl sulfate polyacrylamide gel electrophoresis. formaldehyde was used as chemical treatment, while toasting and ...

The glycoproteins of human erythrocyte membranes were crosslinked with dimethyl adipimidate dihydrochloride. On sodium dodecyl sulfate-polyacrylamide gel electrophoregrams of the crosslinked solubilized membranes, at least three new glycoprotein complexes appeared in addition to the normal glycoprotein species. One of the new glycoprotein complexes was shown to contain two of the three species ...

Heat-labile enterotoxins from Escherichia coli strains of human and porcine origins had identical subunit composition, arrangement, and size in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunological differences recently described by others were shown to reside exclusively in the B subunits.