Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S]methionine and [3H]uridine. At least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was det...

A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of p...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

Ureaplasma urealyticum is an organism considered susceptible to tetracycline. Ten tetracycline-resistant (Tcr) clinical isolates and Tcr serotype 9 were examined. All contained DNA sequences homologous to the streptococcal determinant tetM. They differed from each other on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Southern blots, and immunoblots and appeared to represent differ...

Smooth (S)-lipopolysaccharide (LPS) preparations from reference and field strains of several biovars of Brucella abortus, B. melitensis, and B. suis were prepared by (i) the hot phenol-water method, (ii) hot sodium dodecyl sulfate extraction and proteinase K digestion, or (iii) dimethyl sulfoxide extraction. These S-LPS-enriched fractions were further analyzed by sodium dodecyl sulfate-polyacry...

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a technique that is used to separate proteins in a mixture for the purpose of identifying them individually. Proteins coated in SDS (which gives them a negative charge), are pulled through a thin gel by an electrical current. They separate on the gel based on size--the larger the protein, the slower it moves. Proteins can be stained w...

Heat-labile enterotoxins from Escherichia coli strains of human and porcine origins had identical subunit composition, arrangement, and size in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunological differences recently described by others were shown to reside exclusively in the B subunits.

Ornithine racemase has been purified to homogeneity from Clostridium sticklandii, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is the first racemase known to be highly specific to ornithine. This PLP-dependent enzyme has an M(r) of 92, 000, with a K(m) for L-ornithine of 0.77 +/- 0.05 mM and a k(cat) of 980 +/- 20 s(-1).

The effects of crude plasma and ethanol-extracted swamp eel at different concentrations (0, 0.25, 0.5, 0.75, 1, 1.5, 2 mg/g) as inhibitor protease in the quality tilapia (Oreochromis mossambicus) surimi were investigated. parameters analyzed gel strength, expressible moisture, whiteness, microstructure, protein solubility, hydrolysis pattern by sodium dodecyl sulfate-polyacrylamide electrophore...

Abbreviations : apo, apolipoprotein; CE-OOH, cholesteryl ester hydroperoxides; RP-HPLC, reversed-phase high-performance liquid chromatography; LDL, low-density lipoprotein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TBARS, 2-thiobarbituric acid-reactive substances; TBS, Tris-HCl-buŠered saline; a-Toc, a-tocopherol; VLDL, very-low-density lipoprotein Biosci. Biotechnol....