We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol. 102: 2067-2075) that actin binds with positive cooperativity to plasma membranes from Dictyostelium discoideum. Actin is polymerized at the membrane surface even at concentrations well below the critical concentration for polymerization in solution. Low salt buffer that blocks actin polymerization in solution also pr...

a copolymer of styrene and methyl styrene was synthesized by nitroxide - mediated “living” free radical polymerization )nmp). n-bromosuccinimide was used as a brominating agent to obtain a copolymer with bromine as its end group. this brominated copolymer was used as macro initiators for atom transfer  radical  polymerization  (atrp).  chemical  modifcation  of  the  clay  and transformation of...

in this work, modification of carbon paste electrode surface with poly (2, 5-dimethyl aniline) (p-2,5-dma) by using electrochemical polymerization was described. then, transition metal ions of ni(ii) were incorporated into the polymer film by two ways. at first way, the polymeric modified electrode was immersed in 0.2 m niso4 solutions and the second way was carried out by electrodeposition of ...

A novel method based on in situ surface polymerization of methyl methacrylate (MMA) has been developed for the rapid fabrication of poly(methyl methacrylate) (PMMA) capillary electrophoresis (CE) microchips. MMA containing both thermal and ultraviolet (UV) initiators was allowed to prepolymerize in a water bath to form a fast curing molding solution that was subsequently sandwiched between a ni...

A simple porous gel synthesis method is proposed in this paper. We found that the sequential modification of conventional radical polymerization gives rise to pore formation in acrylamide gels and investigated the parameters that are responsible for controlling the number and the size of pores. Pore formation is dominated by the amount of polymerization initiator (ammonium persulfate), accelera...

Free radical polymerization is one of the most widely employed polymerization techniques. This technique is applied to prepare latexes to be used in paints, high molecular weight poly (methyl methacrylate) for safety glass (Plexiglas), or foamed poly (styrene) to be applied in coffee cups. Some advantages of radical polymerizations, with respect to other techniques, are the relative insensitivi...

Nucleotide polymerization proceeds in the forward (5'-3') direction. This tenet of the central dogma of molecular biology is found in diverse processes including transcription, reverse transcription, DNA replication, and even in lagging strand synthesis where reverse polymerization (3'-5') would present a "simpler" solution. Interestingly, reverse (3'-5') nucleotide addition is catalyzed by the...

Stackable gels comprised of layers of dissimilar polymers were synthesized by combining conventional free radical polymerization (FRP) and atom transfer radical polymerization (ATRP) using two approaches: (i) polymerization of a pre-gel solution containing a monomer and cross-linker introduced on top of a previously prepared gel, and (ii) simultaneous polymerization of two immiscible pre-gel so...

This investigation indicates the ability to selectively graft glycidyl methacrylate (GMA) only from the external surface of regenerated cellulose (RC) ultrafiltration (UF) membranes using activator generated electron transfer (AGET) atom transfer radical polymerization (ATRP). This controlled polymerization resulted in epoxy functionalized polymer brush ends. Further reaction of the terminal ep...