BACKGROUND & OBJECTIVES The streptococcal pyrogenic exotoxins (SPEs) are produced by Streptococcus pyogenes and belong to the family of bacterial superantigens, a group of highly mitogenic proteins. The aim of this study was to search unfinished streptococcal genomes for novel superantigens, to generate recombinant proteins from potential open reading frames (ORFs) and to analyse them for super...

An automated synthesis system using a solid-phase extraction (SPE) system and column packed with octadecylsilica (ODS), which was coated with phospholipid and loaded with dog liver microsomes, was developed for synthesis of glucuronides. Preparation of the microsome-immobilized SPE column, glucuronidation of drugs to synthesize the glucuronides and elution of the products were performed by an a...

A liquid chromatographic method with comfirmation by capillary electrophoresis was used to determine 12 sulfonylurea herbicides in agricultural water. Analysis of 3 different water matrixes fortified at 2 levels gave good recoveries with adequate sensitivity at the 0.1 ppb level. A portion of the water was acidified with acetic acid and loaded onto an RP-102 solid-phase extraction (SPE) cartrid...

It has been shown that streptococcal pyrogenic exotoxin B (SPE B) can induce cells to undergo apoptosis. The present study is to dissect the role of SPE B protease and SPE B protein in the apoptotic process of A549 cells and to elucidate the SPE B-induced apoptotic pathway. Recombinant SPE B (rSPE B) and C192S, a mutant of SPE B without protease activity, were expressed in Escherichia coli and ...

Material and methods Clinically characterized soy allergic patients (n=15) with specific IgE against Gly m 5 and 6 were selected for the study. Here we show the complete data obtained for seven patients. Five chemically characterized soy protein extracts (SPE) were tested: raw SPE, SPE heated (121C) for 10 or 30 minutes with glucose and SPE heated (121C) for 10 or 30 minutes without sugar as a ...

Software-Performance-Engineering (SPE) bezeichnet verschiedene Ansätze zur Performanzverbesserung von Software. Diese wurden bisher nicht vergleichend untersucht. Der vorliegende Beitrag beschreibt ein Vorgehen, um zu überprüfen, inwiefern die SPE-Ansätze die Entstehung performanter Software sicherstellen. Trotz des Vorhandenseins der SPE-Ansätze lassen sich bspw. beim Applikationsserver Apache...

We present two English-to-Czech systems that took part in the WMT 2013 shared task: TECTOMT and PHRASEFIX. The former is a deep-syntactic transfer-based system, the latter is a more-or-less standard statistical post-editing (SPE) applied on top of TECTOMT. In a brief survey, we put SPE in context with other system combination techniques and evaluate SPE vs. another simple system combination tec...

Solid polymer electrolyte (SPE) composed of semi-crystalline poly (vinylidene fluoride-hexafluoropropylene) [P(VdF-HFP)] copolymer, 1-ethyl-3-methylimidazolium bis (trifluoromethyl sulphonyl) imide [EMI-BTI] and graphene oxide (GO) was prepared and its performance evaluated. The effects of GO nano-filler were investigated in terms of enhancement in ionic conductivity along with the electrochemi...

Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment and prevention of diabetes, cardiovascular diseases, chronic asthma and cancer. The recommended dose of this decoction for cancer patients is 500 mL administered orally three times a day. Our approach aimed at reducing the high amount of fluid intake required by fractionation of ASMq guide...

The main purpose of this work was the preliminary qualitative study of organic compounds in wastewaters of swine slaughterhouses. The samples were collected in a local abattoir and submitted to Liquid-Liquid Extraction (LLE) and Solid-phase Extraction (SPE) with XAD-4 resin as stationary phase. The instrumental analysis was performed by Gas Chromatography with Mass Spectrometer Detector (GC/MSD...