Cryopreservation of human sperm, now generally required in donor insemination programs, adversely affects the sperm in terms of standard sperm evaluation parameters and fertilizing ability. The freeze-thaw process appears to produce sublethal damage that appears only after a delay. The authors hypothesized that cryopreservation enhanced peroxidation of sperm membrane lipids, based on previous s...

Despite the significant progress, the post-thaw viability and fertility of the cryopreserved sperm are still reduced, as a consequence of accumulated cellular injuries that arise throughout the cryopreservation process. Many laboratory tests have already been carried out to verify these detrimental effects and their origin. Their is needed to well understand the whole process of cryopreservatio...

background: human sperm cryopreservation has proven to be very valuable. cryopreservation has effects on function, morphology and percentage of fertilization capacity of human sperm. also, it has been revealed that cryopreservation has a role in sperm dna fragmentation and infertility. in this study, viability, motility, dna fragmentation and for the first time, intracellular nitric oxide asses...

Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough in...

The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addi...

OBJECTIVE To review sperm cryopreservation usage rates, corresponding reproductive outcomes, and the current situation in our locality. DESIGN Retrospective case series. SETTING Assisted Reproductive Technology Unit of the Department of Obstetrics and Gynaecology, Prince of Wales Hospital and the Chinese University of Hong Kong. PARTICIPANTS There were 130 Chinese male patients who underw...

Damage occurring to spermatozoa during cryopreservation results in a loss of motile cells and cells that are functionally normal, compared with fresh sperm samples. Treating bull sperm with cholesterol-loaded cyclodextrins (CLC) before cryopreservation results in increased sperm cryosurvival. However, in previous studies, CLC were always added to sperm samples that had been highly diluted. The ...

1. The presence of a large amount of glycoconjugates on the anuran amphibian germ cells was demonstrated using fluorescein isothiocyanate lectins binding specifically to D-galactose and at a lower level, by other lectins binding specifically to iV-acetyl-galactosamine. 2. Glycoconjugates including D-galactose were found near the pseudopodial expansions and in the extracellular space, between ge...

Cryopreservation induces injuries to fish spermatozoa that in turn affect sperm quality in terms of fertilization ability, motility, DNA and protein integrity and larval survival. To reduce the loss of sperm quality due to freezing-thawing, it is necessary to improve these procedures. In the present study we investigated the ability of two antifreeze proteins (AFPI and AFPIII) to reduce the los...