Adequate methods for monitoring any type of gametic mutation directly in man are virtually nonexistent. A method is presented by which one can monitor Y chromosomal nondisjunction directly in the male gamete by quantifying the number of spermatozoa with two fluorescent bodies (YFF) in 1000 sperm counted. Dried semen slides are stained with quinacrine dihydrochloride and examined under a fluores...

BACKGROUND The availability of testis specific genes will be of help in choosing the most promising biomarkers for the detection of testicular sperm retrieval in patients with non-obstructive azoospermia (NOA). Testis specific chromodomain protein Y 1 (CDY1) is a histone acetyltransferase which concentrates in the round spermatid nucleus, where histone hyperacetylation occurs and causes the rep...

BACKGROUND Fatty acid binding proteins (FABPs) are members of the intracellular li- pid binding protein (iLBPs) family and most of them show tissue specific expression. FABP9/PERF15 (Perforatorial15) is the male germ cell-specific fatty acid-binding pro- tein. It was first identified as the major constituent of the murine sperm perforatorium and perinuclear theca. To date, investigations in mic...

OBJECTIVE To compare sensitivity of inner cell mass (ICM) outgrowth assay and analysis of culture media amino acid turnover with the sensitivity of the human sperm motility assay (HSMA) and murine embryo assay (MEA) for detection of formaldehyde toxicity. DESIGN Prospective in vitro study. SETTING University hospital-based infertility center. ANIMAL(S) Murine embryos. INTERVENTION(S) Th...

INTRODUCTION The detection rate and associated factors of at least one sperm in urinary sediment is not well-known in real clinical practice. AIMS The aim of the present study was to evaluate the clinical features associated with the presence of sperm in urinary sediment in a large number of samples. METHODS We conducted a cross-sectional study at Tokyo Saiseikai Central Hospital. We identi...

The selection of offspring from the desired sex can be one of the determining factors to increase the genetic progress and farmer ́s profitability in either beef or dairy cattle. In fact, the use of sex-sorted sperm has been applied worldwide combined with artificial insemination (AI) upon estrus detection in heifers. Additionally, several researches have been performed aiming to investigate the...

casa (computer assisted semen analysis) systems are designed to assist andrologist labour. most available casa systems are not accurate or so expensive. therefore labours use manual methods to provide parameters. although some companies have achieved appropriate accuracy, they have not released their methods. so proposing methods in this area might be useful for groups who intend to design new ...

Chlamydia trachomatis infection is considered to be one of the most common sexually transmitted diseases. It is currently unclear whether chlamydial infection causes pathological conditions of the male accessory glands has consequences for male infertility. To determine the frequency of C. trachomatis Infection among infertile men with leukocytospermia using different diagnostic techniques such...

There is a serious problem with the reduction of male reproductive performance of the livestock in the world. We have a hypothesis that the splicing error-caused derivation of aberrant sperm motility-related proteins may be one of its causal factors. It is thought that fresh testicular tissues are necessary for the detection of splicing errors of the mRNA. However, it is difficult to obtain tes...

OBJECTIVES To obtain knowledge on male reproductive toxicity of inorganic lead at current European exposure levels and to establish lowest adverse effect levels, if any. METHODS A cross sectional survey of the semen of 503 men employed by 10 companies was conducted in the United Kingdom, Italy, and Belgium. The mean blood lead concentration was 31.0 microg/dl (range 4.6-64.5) in 362 workers e...