Semen samples collected from 28 male partners of infertile couples were divided into three equal aliquots and prepared with three selected media, such as PureSperm (Nidacon, Gothenburg, Sweden), Sil-Select Plus (Fertipro, Beernem, Belgium) and SpermGrad (Vitrolife, Gothenburg, Sweden). The differences in mean percentages of semen parameters were assessed by repeated measures analysis. Correlati...

in order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. the semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. the iron and lead content of the seminal plasma (mean ± sem) was recorded as...

The goal of this study was to examine the effect of caffeine on motility parameters of cooling-stored ram sperm – total motility and progressive movement. Caffeine is a cyclic nucleotide phosphodiesterase inhibitor, which can affect sperm motility. The semen was kept at 4 ̊C for several days. Caffeine was added to fresh ram semen diluted in Triladyl at concentrations of 1, 2 and 4 mmol.l -1 ever...

the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from ...

The present study to investigate that the correlation between sperm parameters and sperm DNA fragmentation in fertile and infertile cigarette smoking patient. Forty men undergoing infertility evaluation were divided into four groups according their smoking ability (A: 2 days ( n=10), B: 4 days ( n=10), C: 8 days ( n=10), D: 15 days ( n=10)) sperm parameters ( sperm concentration, pH, Viscosity,...

The combination of laser tweezers, fluorescent imaging, and real-time automated tracking and trapping (RATTS) can measure sperm swimming speed and swimming force simultaneously with mitochondrial membrane potential (MMP). This approach is used to study the roles of two sources of ATP in sperm motility: oxidative phosphorylation, which occurs in the mitochondria located in the sperm midpiece and...

The objectives of this study were to investigate the effects of testis-epididymis cool storage on viability and progressive motility of buffalo epididymal sperm (EP) and to compare the influences of two basic semen extenders on post thaw EP viability and progressive motility. Abattoir collected buffalo testicles were allotted to three storage times (0 h: n = 10; 24 h: n = 10 and 48 h: n = 12). ...

The effects of acute restraint stress on sperm motility and reproductive endocrinology were investigated in adult rats. Sperm motility was determined by computer-assisted sperm analysis. Acute restraint stress reduced sperm motility starting after 30 min, and the sperm motility parameters, percentage of motile spermatozoa (%), straight-line velocity, curvilinear velocity, deviation of the sperm...

background: lipid peroxidation is known to cause various impairments to sperm cells and may play a major role in the etiology of male infertility. asthenospermia is the main factor of male infertility and has significantly higher level of peroxidation than in normozoospermic males. materials and methods: using thiobarbituric acid (tba) assay procedure, we have determined the level of lipid pero...