Hybridomas secreting monoclonal antibodies have been produced by fusion of NS-1 mouse myeloma cells with the spleen cells of mice inoculated with a 60-65,000-mol wt fraction of proteins released from Drosophila embryo nuclei treated with DNase I. The antibodies secreted by the hybridomas were examined with polytene chromosomes of formaldehyde-fixed salivary gland squashes by an immunofluorescen...

THIS STUDY WAS DESIGNED TO ANSWER THE QUESTION: Is H(3)-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H(3)-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered wit...

FRIENDS WITH BENEFITS; NATURAL ENEMIES AND POLLINATORS IN VIRGINIA CUCURBIT PRODUCTION. James M. Wilson & Thomas P. Kuhar, Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061. Cucurbit production in Virginia provides the commonwealth with melons, pumpkins, summer squashes, and cucumbers, all of which are dependent upon adequate pollination for fruit set and development. Growers ...

BACKGROUND A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005-2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other virus/vector combinations. METHODOLOGY AND FINDINGS We orally infected 8 Ae. albopictus collections from La Réunio...

The origin of agriculture was a signal development in human affairs and as such has occupied the attention of scholars from the natural and social sciences for well over a century. Historical studies of climate and vegetation are closely associated with crop plant evolution because they can reveal the ecological contexts of plant domestication together with the antiquity and effects of agricult...

BACKGROUND A prospective cohort study of 2512 Welshmen aged 45-59 living in Caerphilly in 1979-1983 was used to investigate associations between diet and lung function. METHODS At baseline (phase I) and at five year follow up (phase II), forced expiratory volume in one second (FEV(1)) was measured using a McDermott spirometer and dietary data were obtained using a semi-quantitative food frequ...

a r t i c l e i n f o The bees of the Peponapis genus (Eucerini, Apidae) have a Neotropical distribution with the center of species diversity located in Mexico and are specialized in Cucurbita plants, which have many species of economic importance, such as squashes and pumpkins. Peponapis fervens is the only species of the genus known from southern South America. The Cucurbita species occurring...

Polytene chromosomes from the salivary gland cells of Drosophila third instar larvae have been used extensively for cytological and genetic studies. Polytene chromosomes have also been used to study transcription because an actively transcribing gene is often manifested as a localized region of chromatin decondensation or “puff”. Several methods have been developed to detect sites of active tra...

Autofluorescence has been reported as a method for detecting oocysts of Cyclospora cayetanensis in patients with seasonal diarrhoea (Berlin O.G.W., Peter J.B., Gagne C., Conteas C.N., Ash L.R. 1998: Emerg. Inf. Dis. 4: 127-128), although the same technique had been used earlier, for probably the same parasite, in human subjects with prolonged diarrhoea (Long E.G., White E.H., Carmichael W.W., Q...