Objective: The aim of the present study was to develop a reversed phase high-performance liquid chromatography (RP-HPLC) method for simultaneous estimation of betulin, lupeol and stigmasterol in Asteracantha longifolia extract. Methods: The chromatographic separation was achieved using Luna C18 column (5 μm, 250 x 4.6 mm) under isocratic elution of acetonitrile and 0.1% acetic acid in water (94...

The dietary impact of specific phytosterols incorporated into phytosterol fatty acid esters has not been elucidated. Therefore, we tested the hypothesis that phytosterol esters containing different sterol moieties (sitosterol, sitostanol, or stigmasterol) but the same fatty acid moiety (stearic acid) produce different effects on cholesterol metabolism. Male Syrian hamsters were fed sitosterol, ...

The effect of changes of membrane fluidity on the oxygen evolving capability of isolated thylakoids was investigated. Alteration of the lipid phase fluidity was achieved by incorporation of the plant sterol stigmasterol. Incorporation of stigmasterol in the lipid bilayer of thylakoid membranes results in rigidization of the hydrophobic phase of thylakoid membranes and decreases the degree of pa...

Sterols play a key role in various physiological processes of plants. Commonly, stigmasterol, β-sitosterol and campesterol represent the main plant sterols, cholesterol is often reported as trace sterol. Changes especially β-sitosterol/stigmasterol levels, can be induced by different biotic abiotic factors. Plant parasitic nematodes, such root-knot nematode Meloidogyne incognita, are devastatin...

Aims: To study the anti-HIVs and anti-bacterial activity investigate chemical compositions of crude extracts isolated compounds from stems twig Ficus dubia.
 Methodology: Twigs dubia were collected Si Sawat District, Kanchanaburi Province, Thailand. Bioactivity assay phytochemical analysis ficus processed under standard method including, anti-HIV1-RT, antibacterial activities, CC chromatog...

Structural properties of paeonol-encapsulated liposomes containing cholesterol or stigmasterol at 37°C have been investigated by synchrotron small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) techniques. We compared the structural properties of pure dipalmitoylphosphatidylcholine (DPPC) liposomes, sterol–DPPC liposomes, and those of paeonol–sterol–DPPC liposomes at diffe...

The identity, composition, and concentration of the total, free, esterified, and glycosidic sterol fractions were determined during germination of tobacco seeds. The total, free, and esterified sterols increased, with stigmasterol and campesterol accounting for most of the increase. Steryl glycosides decreased during germination, and stigmasteryl and sitosteryl glycosides showed the largest dec...

The phytochemical study of Sida rhombifolia L. (Malvaceae) led to the isolation through chromatographic techniques of eleven secondary metabolites: sitosterol (1a) and stigmasterol (1b), sitosterol-3-O-b-D-glucopyranoside (2a) and stigmasterol-3-O-b-D-glucopyranoside (2b), phaeophytin A (3), 17³-ethoxypheophorbide A (4), 13²-hydroxy phaeophytin B (5), 17³-ethoxypheophorbide B (6), 5,7-dihydroxy...