نتایج جستجو برای: suppression subtractive hybridization

تعداد نتایج: 160708  

Journal: :Methods in molecular biology 2009
Andrew Hillmann Eimear Dunne Dermot Kenny

The comparison of two RNA populations that differ from the effects of a single-independent variable, such as a drug treatment or a specific genetic defect, can identify differences in the abundance of specific transcripts that vary in a population-dependent manner. There are a variety of methods for identifying differentially expressed genes, including microarray, SAGE, qRT-PCR, and DDGE. This ...

Journal: :Acta biochimica et biophysica Sinica 2008
Zhongyuan Jin Baoan Liu Deyun Feng Chen Chen Xiang Li Yongbin Hu Jinwu Peng Yu Liu Jing Du Chunyan Fu Jifang Wen

The critical molecular mechanism in the development of the pulmonary fibrosis remains unknown, leaving diagnosed patients with a poor prognosis. To isolate the genes specifically up-regulated in pulmonary fibrosis, we established a rat silicosis model 360 d after treatment with crystalline silica suspension. Radiographs of chests showed that some scattered high-density shadows appeared in the l...

Journal: :The Biochemical journal 2004
Ren Lai Lee O Lomas Jan Jonczy Philip C Turner Huw H Rees

Two non-cationic defensin-like antimicrobial peptides, named Amblyomma defensin peptide 1 and Amblyomma defensin peptide 2, were identified from the hard tick, Amblyomma hebraeum, by a combination of suppression subtractive hybridization for differentially expressed genes and proteomics. cDNA clones encoding each of these two defensin-like antimicrobial peptides were isolated from the different...

Journal: :Journal of medical entomology 2010
Liming Zhao James J Becnel Gary G Clark Kenneth J Linthicum Jian Chen Xixuan Jin

Magnesium is crucial for baculovirus transmission in Culex nigripalpus (Theobald) and Culex quinquefasciatus (Say) larvae. However, the mechanistic role of magnesium in baculovirus transmission is unknown. To investigate the possible role of host response factors in baculovirus transmission, suppression subtractive hybridization was used to identify genes differentially transcribed after magnes...

Journal: :Acta histochemica 2011
Sarah E Henson Travis Morford Mary-Pat Stein Randolph Wall Cindy S Malone

Mantle cell lymphoma and small lymphocytic lymphoma are lymphocyte cancers that have similar morphologies and a common age of onset. Mantle cell lymphoma is generally an aggressive B cell lymphoma with a short median survival time, whereas small lymphocytic lymphoma is typically an indolent B cell lymphoma with a prolonged median survival time. Using primary tumor samples in bi-directional supp...

Journal: :Plant physiology 2007
Marçal Soler Olga Serra Marisa Molinas Gemma Huguet Silvia Fluch Mercè Figueras

Cork (phellem) is a multilayered dead tissue protecting plant mature stems and roots and plant healing tissues from water loss and injuries. Cork cells are made impervious by the deposition of suberin onto cell walls. Although suberin deposition and cork formation are essential for survival of land plants, molecular studies have rarely been conducted on this tissue. Here, we address this questi...

2014
Yongzhi LUN Chongbo XU Qing CHI Xuelei WANG Wen SUI Sujuan JIANG

BACKGROUND Hepatitis B Virus (HBV) DNA polymerase transactivated protein 1 (HBVDNAPTP1) is a novel protein transactivated by HBV DNA polymerase, screened by suppression subtractive hybridization technique (GenBank accession no: AY450389). The biological function of HBVDNAPTP1 was investigated in this study. METHODS We constructed a vector pcDNA3.1 (-)/myc-His A-HBVDNAPTP1 and used it to trans...

2017
Indrani K. Baruah Debashis Panda Jagadale M.V Deba Jit Das Sumita Acharjee Priyabrata Sen Bidyut Kumar Sarmah

Black gram (Vigna mungo) seeds are a rich source of digestible proteins, however, during storage these seeds are severely damaged by bruchids (Callosobruchus spp.), reducing seed quality and yield losses. Most of the cultivated genotypes of black gram are susceptible to bruchids, however, few tolerant genotypes have also been identified but the mechanism of tolerance is poorly understood. We em...

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