Transport of thyroid hormone across the cell membrane is required for its action and metabolism. Recently, a T-type amino acid transporter was cloned which transports aromatic amino acids but not iodothyronines. This transporter belongs to the monocarboxylate transporter (MCT) family and is most homologous with MCT8 (SLC16A2). Therefore, we cloned rat MCT8 and tested it for thyroid hormone tran...

BACKGROUND Although the association of clinical hypothyroidism with cognitive deficits is well known, the cognitive effects of thyroid hormones in euthyroid subjects are less studied and understood. The purpose of this study was to examine thyroid-cognition relationships in healthy, euthyroid older men. METHODS We examined healthy men (N = 44, mean age = 72), excluding clinically hypothyroid/...

X 1010 sites/100 ,ug protein, and the other with an apparent Kd of 25 nM and a binding capacity of 1.4 X 1012 sites/100 ,g protein. Measurement of the ability of several thyronine analogues to inhibit the binding of [1251I]T3 revealed the following rank order of potency: L-T3 > L-T4 > D-T3 = D-T4 > L-3,5-T2 > rT3 > D,Lth*ronine. Binding of T3 was inhibited by the omission of calcium from the me...

BACKGROUND α-Tocopherol (α-T) and α-tocotrienol (α-T3) are well recognized as lipophilic antioxidants. Nevertheless, there is limited knowledge on their location in heterogeneous cell membranes. We first investigated the distribution of α-T and α-T3 to the cholesterol-rich microdomains (lipid rafts and caveolae) of heterogeneous cell membranes by incubating these antioxidants with cultured mous...

Chitosan was cross-linked using glutaraldehyde in the presence of magnetite. The resin obtained was chemically modified through the reaction with tetraethylenepentamine followed by glycidyl trimethylammonium chlorid to produce chitosan bearing amine (R1) and chitosan bearing both amine and quaternary ammonium chloride moieties (R2), respectively. The quaternary amine resin (R2) showed a higher ...

In many horses with proximal suspensory desmitis, radiopharmaceutical uptake (RU) in the proximal aspect of the third metacarpal (metatarsal) bone is normal. Increased RU reflects an enthesopathy, which may influence both treatment and prognosis. Authors’ address: Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK; e-mail: [email protected]...

We determined appropriate temperatures for sample storage and the resulting stability of 14 analytes commonly radioimmunoassayed in the clinical laboratory. Serum specimens to be tested for concentrations of cholylglycine, cortisol, digoxin, ferritin, follitropin, immunoglobulin E, lutropin, prolactin, thyroxin (also blood-spot thyroxin), triiodothyronine, and triiodothyronine uptake could be s...

The effects of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF alpha) on basal and TRH-induced TSH release, and the effects of IL-1 beta on the uptake of [125I]T3 and [125I]T4 and on nuclear binding of [125I]T3 were examined. Furthermore, the release of other anterior pituitary hormones in the presence of IL-1 beta was measured. Anterior pituitary cells from male Wistar rats...

The purpose of this study was to investigate the effect of different surface treatments on shear bond strength of saliva-contaminated resin-resin interfaces. Flat resin surfaces were fabricated. In the control group, no contamination or surface treatment was performed. The resin surfaces of the experimental groups were contaminated with saliva and air-dried, and then submitted to: (G1) rinsing ...