The present paper introduces the steel production and BOF slag processing techniques in Steelmaking Sector of Bayuquan Iron and Steel Subsidiary, Ansteel. The technology of slag tailings utilization has been developed under the consideration of the properties of slag tailing and the characteristics of slag forming in BOF steelmaking operation practice. The processing of the BOF slag includes ho...

The Internet’s influence in creating e-services has been revolutionary for retailer. Clearly, The Internet is fast becoming an important new channel for businesses in retailing, raising e-services as the emergent business paradigm in the industrialized world. Unfortunately, past research on measuring e-tailing quality is scarce. This paper provides a conceptual framework for e-tailing quality a...

Online restricted access media with liquid chromatography and tandem mass spectrometry for the direct analysis of small molecules in biological fluids represents an interesting alternative to time-demanding traditional sample preparation techniques. In this study, important considerations concerning the development of a restricted access media with liquid chromatography and tandem mass spectrom...

Pfu DNA Polymerase(a)-generated blunt-end PCR fragments can be ligated into the pGEM®-T and pGEM®-T Easy Vectors(b,c) if the fragments are first A-tailed using Taq DNA Polymerase(a). We describe a method for A-tailing these PCR fragments and demonstrate its utility by cloning two different Pfu DNA Polymerase-generated fragments into the pGEM®-T Easy Vector, using the new 2X Rapid Ligation Buffe...

Method comparison studies of two different methods for the analysis of weak acid dissociable (WAD) cyanide revealed analytical flaws and/or matrix interference problems with both procedures. EPA "draft" method 1677 using a Perstorp 3202 CN analyzer was compared to Standard Method 4500 CN I. It was discovered that the Perstorp analyzer produced more precise and more accurate results once appropr...

In this paper, the phenomenon of electronic retailing is examined. Elements that make e-tailing especially attractive to consumers are highlighted. It is argued that traditional retailers have to critically consider the migration to a digital domain, and address challenges that might impede a smooth transition. Conversely, e-tailers have much to learn from their counterparts in the physical wor...

This scientific review presents the processes of regulation miRNA content. To write article, information was searched using Scopus, Web Science, MedLine, PubMed, Google Scholar, EMBASE, Global Health, The Cochrane Library, CyberLeninka databases. article characteristics microRNA editing and tailing, which regulate content through transcription control. It is emphasized that most important mecha...

We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.

Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.

The legacy of uranium production threatens the environment and public security in Kyrgyzstan and neighboring countries. The key ecological problems include: pollution of the environment with radionuclides; increasing risk of RAW tailing dumps destruction due to climate change, natural disasters and anthropogenic catastrophes which are typical for mountainous and seismic regions, and large land ...