Two-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of ...

Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of an...

Here we describe a 5-step sequence to prepare the AB enone 1, the key precursor to fully synthetic tetracyclines, that begins with a diastereoselective Michael-Claisen coupling of two simple starting materials, a cyclohexenone (compound 2 or, in a refinement, a substituted variant, vide infra) and the isoxazole ester 3. This advance defines an 8-step linear sequence to 6-deoxytetracycline antib...

BACKGROUND Topical corticosteroid (TCS) treatment is widely prescribed for atopic dermatitis (AD). However, TCS treatment is associated with tachyphylaxis, and discontinuation after long-term use may cause exacerbation of symptoms. Some AD patients are reluctant to use TCS. OBJECTIVE To evaluate patient-reported short- and long-term outcomes after discontinuation of TCS treatment for AD. ME...

In a recently published report, Poole et al. utilized a crossover design to determine the effect of nonprescription antimicrobial exposure on the human microbiome (1). After an initial washout phase, participants were provided either products that contained TCS or products without TCS for use over a 4-month period, followed by a switch to the opposite exposure regimen for another 4 months. Typi...

The hazard posed by tropical cyclones (TCs) is often quantified in terms of the minimum central pressure (MCP) or maximum wind speed. While significant, these qualities must be balanced against considerations for the strength and spatial extent of the outer-core circulation, which also significantly determines the total peril posed by these storms. To this end, the results of recent studies on ...

Telocytes (TCs) are interstitial cells that are present in numerous organs, including the heart interstitial space and cardiac stem cell niche. TCs are completely different from fibroblasts. TCs release extracellular vesicles that may interact with cardiac stem cells (CSCs) via paracrine effects. Data on the secretory profile of TCs and the bidirectional shuttle vesicular signalling mechanism b...