We describe a homogeneous enzyme immunoassay for measurement of cannabinoid metabolites, as well as delta9-tetrahydrocannabinol (I) in urine. Malate dehydrogenase from pig heart mitochondria was labeled with a derivative of I. The compound used to calibrate the assay was the I metabolite, 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (II). With 15 microgram of II per liter of urine as th...

1 A'-trans-tetrahydrocannabinol, (A'-THC) produces bronchodilatation in asthmatic patients. 2 Administered in 63 jl metered volumes containing 50-200 jg by inhalation from an aerosol device to patients judged to be in a steady state, it increased peak expiratory flow rate (PEFR) and forced expiratory volume in 1 second (FEVI). 3 The rate of onset, magnitude, and duration of the bronchodilator e...

In Experiment 1, 20 college students learned two identity conditional discriminations using squares that differed in interior-fill percentage (called Fill23 and Fill77). A two-choice generalization test was then presented with number of test trials varied across groups of subjects. The test samples were 19 squares that ranged in fill value from 23% to 77%; the comparisons were squares with Fill...

Sarafian, Theodore A., Shaghig Kouyoumjian, Farnaz Khoshaghideh, Donald P. Tashkin, and Michael D. Roth. 9-Tetrahydrocannabinol disrupts mitochondrial function and cell energetics. Am J Physiol Lung Cell Mol Physiol 284: L298–L306, 2003. First published October 25, 2002; 10.1152/ajplung.00157.2002.—We have observed rapid and extensive depletion of cellular energy stores by 9-tetrahydrocannabino...

Male albino rats, injected intravenously or intracerebroventricularly with delta 1-tetrahydrocannabinol (THC), develop mudriasis. The median effective dose of the intravenous administration group was 5 mg/kg THC, whereas that for the intracerebroventricular route was 150 microgram/kg THC. Sympathectomy significantly decreased the THC-induced mydriasis. The mydriatic effect was not influenced by...

Although   the nutrient composition of hemp products provides evidence that these potentially serve as valuable livestock feed ingredients and may enhance human health, cultivation was prohibited due to high content Δ-9 tetrahydrocannabinol (THC). Recently, regulatory changes by several countries allowed industry under a license permits plants plant parts genera Cannabis with TH...

Ahstn1~~-Pnssngc and trnnsit time of ndult upstream-migrant Amcricnn shnd Alosu sapidissima ~ n d blucback hcrring A. ucstivalls wcrc investigated in standard Dcnil and Alnska stecppass fishways with vnrinhlc slopc and hcndpond undcr scmicontrollcd condition^. Percent of Amcricnn hhad passcd per unit time (percent pnssnge) incrcnsed with temperature, while time required to lrom the lishway entr...