bread-making quality in hexaploeid wheat is a complex trait for breeding programs. high molecular weight glutenin subunits (hmw-gs) have an important effect on dough stability and elasticity. the glu-1 loci that are encoded these subunits are located on the long arms of group-1 homologues chromosomes of the a, b and d genomes. in this work we used pcr-based dna markers to distinguish wheat brea...

10 accessions of tetraploid wheat were radiated with 100 Gy (60)Co γ -ray. The germination energy, germination rate, special characters (secondary tillering, stalk with wax powder, and dwarf), meiotic process, and high-molecular-weight glutenin subunits (HMW-GSs) were observed. Different species has different radiation sensibility. With 1 seed germinated (5%), T. dicoccum (PI434999) is the most...

Genetic transformation via the biolistic method has been used to introduce genes encoding natural and novel high-molecular-weight glutenin subunits (HMW-GS) into wheat. The appearance of new seed proteins of sizes not predicted by the transgene coding sequences was noted in some experiments. In this report, the identities of thirteen of these novel proteins were determined by tandem mass spectr...

to determine ancient seed species, 3250-3450 year-old charred seeds obtained from different iranian archaeological sites were studied using scanning electron microscopy (sem) and molecular analysis. sem analysis of ancient seeds revealed that the surface of the seeds was damaged. therefore, we could not accurately identify their species. molecular analysis on ancient specimens was done on diffe...

Glu-1Ay, one of six genes encoding a high molecular weight glutenin subunit (HMW-GS), is frequently silenced in hexaploid common wheat. Here, an active allele of Glu-1Ay was integrated from wild emmer wheat (Triticum turgidum ssp. dicoccoides) accession D97 into the common wheat (Triticum aestivum) cultivar Chuannong 16 via the repeated self-fertilization of the pentaploid interspecific hybrid,...

Based on the electrophoretic spectrum, analysis, 216 genotypes of spring wheat were identified as belonging to 25 types including 18 types of HMW-glutenin for 188 homogeneous cultivars. Most of the spring wheat KASIB could be characterized by the subunits 2 * 7+9 5+10 (40% genotypes), and subunits 2* 7+9 2+12– (30%) and subunits 1*7+9 5+10– (8%). The diversity appears to be determined by varyin...

To better understand the transcriptional regulation of high molecular weight glutenin subunit (HMW-GS) expression, we isolated four Glu-1Bx promoters from six wheat cultivars exhibiting diverse protein expression levels. The activities of the diverse Glu-1Bx promoters were tested and compared with β-glucuronidase (GUS) reporter fusions. Although all the full-length Glu-1Bx promoters showed endo...