We investigated the structure of bacterial communities present in livestock manure-based composting processes and evaluated the bacterial succession during the composting processes. Compost samples were derived separately from swine manure, dairy manure and sewage sludge. The structure of the bacterial community was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (...

We used classical molecular dynamics simulation method to investigate physical factors responsible for the increased thermal stability of proteins from thermophilic and hyperthermophilic organisms. Subject of investigation were two pairs of homologous proteins from the functional classes of: 1) cold shock proteins from Escherichia coli (mesophilic) and Bacillus caldolyticus (thermophilic) and 2...

The microflora of milk powder consists of a wide array of microorgan­ isms of which special attention is given to Bacillus endospores. Bacillus endospores survive pasteurization and spray drying and inhabit the final powder product in the dormant state indefinitely. Once the powder is re­ constituted, endospores may germinate, and through their enzymatic ac­ tivity become detrimental to quality...

The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucidate the physical basis of the difference in stability, previously developed th...