A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold better than other thermostable DNA polymerases. Since the enzyme also catalyzes pyrophosphorolysis at dideoxy termini, a thermostable inorganic py...

Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure...

Thermostable composite materials based on solid magnesium phosphate and calcium phosphate, as well hybrid binders have been developed investigated. Thermal phase transformations of the composites studied. Strength characteristics determined in temperature range 20–1000 °C. It is shown that obtained high strength properties (compressive reaches 120–130 MPa) are characterised by thermal stability...

Background: In recent years, ionic liquids and enzymes have been widely used in the separation and extraction processes of natural products. Chlorogenic acid (CGA) has important biological and pharmacological activities. It is significant to develop a green and efficient method to extract GCA from Flos Lonicera japonica (FLJ) by integrating the advantages of the ionic liquids and enzymes. Resul...

Variation in the ability of Aspergillus flavus isolates to spread between cotton boll locules was previously shown to be at least partially related to variation in the production of a specific polygalacturonase (pectinase P2C). To determine if non-pectolytic hydrolase differences between low- and high-virulence isolates exist and, thus, could also potentially contribute to virulence differences...

Apricots (Prunus armeniaca L.), a kind of climacteric fruits, are favored by consumers for their flavor and texture, but they are highly perishable. Apricots soften rapidly after harvest. Once softened, they are easily damaged such as latent damage, which limits their marketing time [1, 2]. Therefore, a common practice for handling and storing apricots is early harvest and applying postharvest ...

Out of various industrial enzymes, thermostable βglucosidases are of great interest, due to their utility in conversion of lingocellulosic material into bioethanol. Determination of the factors responsible for thermostability in β-glucosidases by sequence and structure comparison will be useful in identification, isolation and design of novel thermostable βglucosidases. In the present study the...

Previously identified fungal pectinase producers of the species Aspergillus sojae were used for optimization of polygalacturonase production in solid-state fermentation applying Design of Experiment. The effects of media composition and several process parameters, like inoculum size, moisture level, incubation time and temperature on polygalacturonase activity were studied in screening and opti...

The demand for enzymes in the global market is projected to rise at a fast pace in recent years. There has been a great increase in industrial applications of pectinase owing to their significant biotechnological uses. For applying enzymes at industrial scale primary it is important to know the features of the enzyme. Thus, this study was undertaken with aims of characterizing the pectinase enz...