The superfamily cytochrome P450s is involved in the evolution of insecticide resistance. However, whether CYP4G68, a differentially expressed gene identified from our transcriptomics analysis, confers resistance to world’s heavily used class neonicotinoids unknown. Hence, we explored role CYP4G68 conferring imidacloprid and thiamethoxam Bemisia tabaci. species B. tabaci MED developed low-to-hig...

Many previous studies report nectar residues being consistently lower than pollen residues in the same plant species. This is also evident in our samples from the oilseed rape (OSR) crop, where 100% of pollen samples contained thiamethoxam, whereas only 53.9% of the nectar samples had detectable levels of this compound. However, as clearly explained in our manuscript, our wildflower nectar and ...

Soybean aphid is an economic pest of soybean in North America. Currently, management of soybean aphid is achieved through the use of foliar- and seed-applied insecticides. However, natural enemies play an important role in regulating soybean aphid populations, and may be adversely affected by insecticides. The effects of imidacloprid and thiamethoxam seed treatments on the soybean aphid parasit...

A field experiment was conducted to study the rate of dissipation thiamethoxam 25% WG in vegetable cowpea. Thiamethoxam 25%WG @ 0.24 ml l-1 sprayed at pod formation stage, and samples pods were harvested 0 (2 hr after spray), 1, 3, 5, 7, 10, 15 days spray. Residues estimated using liquid chromatography tandem mass spectrometer (LC-MS/ MS) All-India Network Project on Pesticides Residues, Colleg...

A method for the determination of Thiamethoxam in bee samples was set up by means of high performance liquid chromatography with an electrochemical detector and post-column photochemical reactor (HPLC-h nu-ED). Analytical method was based on a rapid sample extraction procedure with acetone, followed by chromatographic separation into a C18-RP column isocratically operated by 60 mM phosphate buf...

The use of neonicotinoid seed treatments is a nearly ubiquitous practice in sunflower (Helianthus annuus) pest management. Sunflowers have a speciose pest complex, but also harbor a diverse and abundant community of beneficial, nontarget organisms which may be negatively affected by pest management practices. Here, we investigate how the foliar and subterranean arthropod pest communities in sun...

Field-based bioassays and residue profile analysis were used to determine the relative toxicity, rainfastness, and field degradation over time of five insecticides from five insecticide classes on adult Japanese beetles, Popillia japonica Newman (Coleoptera: Scarabaeidae), in grapes, Vitis labrusca L. Bioassays assessed Japanese beetle condition as alive, knockdown, or immobile when exposed for...

Two experiments were conducted at the Delta Research and Extension Center in Stoneville, MS, during 2011 and 2012 to determine the impact of water management practices on the efficacy of insecticidal seed treatments targeting rice water weevil, Lissorhoptrus oryzophilus Kuschel. Larval densities and yield were compared for plots treated with labeled rates of thiamethoxam, chlorantraniliprole, a...

Acute toxicity of commonly used insecticides viz., fipronil, cyantraniliprole, fluxametamide, phenthoate and thiamethoxam were determined against Red pumpkin beetle, Aulacophora foveicollis collected from the unsprayed fields in orchard Tamil Nadu Agricultural University, Coimbatore using filter paper disc bioassay method. Based on LC50 values (ppm) different insecticides, order was found to be...

Whilst a formal statistical analysis of any experimental data is always preferable in principle, in the case of Pilling et al. (PLoS ONE 8:e77193, 2013), it is hard to see how the results of any formal analysis-including those provided by Schick et al.-could be considered reliable. Regardless of the issue of statistical analysis, there was a wealth of valuable and novel biological and chemical ...