نتایج جستجو برای: thiocarbohydrazide
تعداد نتایج: 77 فیلتر نتایج به سال:
Previous studies on the fractionation of human neutrophil granules have identified two major populations: myeloperoxidase (MPO)-containing azurophil, or primary, granules and MPO-deficient specific, or secondary, granules. Peripheral blood neutrophils from individual donors were lysed in sucrose-free media by either hypotonic shock or nitrogen cavitation. Using a novel two-gradient Percoll dens...
Problem of three-dimensional reconstruction of cellular structures with high level of resolution along X, Y and especially Z axes remains one of the most difficult tasks of the modern electron microscopy. For 3D microscopy one could use SEM or SEM tomography. However, for structure inside cells the problem of surface dirt is not completely solved. For 3D reconstruction of cellular organelles, t...
PURPOSE Dietary fat intake has been proposed as a mechanism of sub-RPE deposit formation. It has been demonstrated recently that sub-RPE deposits develop in 16- to 18-month-old C57BL/6 mice fed a high-fat diet and exposed to blue-green light. Hyperlipidemia also develops in these mice after they consume a high-fat diet. Because hyperlipidemia also develops in young C57BL/6 mice that overexpress...
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Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle in human cells. We present four examples of uses for this workflow which are not practical by light microscopy and/or transmission electron micros...
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