Tuberculous meningitis (TBM) is a severe form of extrapulmonary tuberculosis. The aims of this study were to evaluate in-house molecular diagnostic protocols of DNA extraction directly from CSF samples and the targets amplified by qPCR as an accurate and fast diagnosis of TBM. One hundred CSF samples from 68 patients suspected of TBM were studied. Four DNA extraction techniques (phenol-chlorofo...

Glucosinolates (GLSs) are abundant in cruciferous vegetables and reported to have anti thyroidal effects. Four GLSs (sinigrin, progoitrin, glucoerucin, and glucotropaeolin) were administered orally to rats, and the breakdown products of GLSs (GLS-BPs: thiocyanate ions, cyanide ions, organic isothiocyanates, organic nitriles, and organic thiocyanates) were measured in serum. Thiocyanate ions wer...

KINSEY AND PALM (1955) have described the kinetics of penetration of two ions, sodium and thiocyanate, into the aqueous humour, and have concluded that there is a fundamental difference in their modes of penetration; the thiocyanate ion, contrary to an original claim (Kinsey and Grant, 1942), penetrating by " diffusion ", whilst sodium is said to penetrate by " secretion". An inspection of thei...

I show how mercuric thiocyanate reagent can be used in assaying serum chloride economically, rapidly, and precisely. I used a semiautomated discrete analyzer with direct concentration read-out capability but the method can easily be applied to many mechanized instruments. Linearity between concentration and color is obtained by adding appropriate amounts of mercuric nitrate to the reagent. As m...

Abstract A method for rapid separation of americium from complex matrices by use two commercially available extraction chromatography resins is reported. TRU resin capable purifying americium/lanthanides together Group 1, 2 and transition metals. tolerated high loadings iron, aluminium, calcium sodium potassium. TEVA purified elution with ammonium thiocyanate. Decontamination factors > 20,00...

We describe a procedure for determining thiocyanate in plasma with a Technicon AutoAnalyzer I, modified with a 50-mm tubular flow cell to increase the sensitivity of thiocyanate detection. The procedure is suitable for distinguishing cigarette smokers from non-smokers, the mean for smokers being 151 (SD 43) micromol/L and for non-smokers 62 (SD 19) micromol/L.

A study of the inhibition of the growth of Streptococcus cremoris 972 by the enzyme lactoperoxidase has shown, in agreement with previous investigations, that the inhibition requires a source of both peroxide and thiocyanate. The thiocyanate may play more than one role. It stabilizes the very dilute solutions of lactoperoxidase employed in these studies, and its oxidation products may be involv...

OBJECTIVES Hypothyroidism in utero leading to mental retardation is highly prevalent and recurrent in developing countries where iodine deficiency and thiocyanate overload are combined. So, to explore and identify human population's risks for developing iodine deficiency disorders and their endemicity in Western Cameroon, with the aim to prevent this deficiency and to fight again it, urinary io...

a carbon ionic liquid electrode (cile) modified with tio2-fe nanoparticles was constructed by incorporating tio2-fe nanoparticles into the paste matrix. under the optimized experimental conditions, using differential pulse voltammetry (dpv), the oxidation of thiocyanate and oxalate were occurred at potentials of 0.740 v and 1.010 v, respectively, at the surface of the modified electrode. observi...

To evaluate the susceptibility of steels to hydrogen embrittlement, it must be charged and have its content controlled before any mechanical testing. In this study, permeation tests an iron sheet were performed during potentiostatic charging in various solutions containing ammonium thiocyanate obtain a guideline for efficient wide range contents. As polarization potential shifted negative direc...