Traditional protein chips are based on solid and cannot enable drug target screening in a label-free manner. Herein, thermal shift chip (PTSC) fluorescence signals is proposed, which enables low-cost, high-throughput, screening. We developed PTSC for COVID-19, containing 12 SARS-CoV-2 host proteins. A series of quality-control tests were performed small-molecule drugs, macromolecular antibodies...

Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated with significant and recent examples. These high throughput strategies are also discussed with an emp...

The last decade has seen significant progresses in the application of combinatorial approaches and high-throughput screening in photocatalyst discovery. This paper aims at providing a comprehensive review on the parallel synthesis and high-throughput characterization of photocatalysts, including the development of instrumentation, strategy of experiment, preparation of libraries, high-throughpu...

The trend towards assay miniaturization for high-throughput and ultra-high-throughput screening continues to spur development of homogeneous, fluorescence-based assays in higher density, smaller volume microplate formats. Recently, first-generation microfluidic devices have been designed for performing continuous-flow biochemical and cell-based assays. These devices provide orders-of-magnitude ...

HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ending with the discovery of lead compounds. ...

BACKGROUND Antimicrobial resistance is a great concern in the medical community, as well as food industry. Soy peptides were tested against bacterial biofilms for their antimicrobial activity. A high throughput drug screening assay was developed using microfluidic technology, RAMAN spectroscopy, and optical microscopy for rapid screening of antimicrobials and rapid identification of pathogens. ...

In many cases, optimal operation for a plant is the same as maximum throughput. In this case a rigorous model for the plant is not necessary if we are able to identify the bottleneck. Optimal operation is the same as maximum throughput in the bottleneck. If the bottleneck does not move, this can be realized with singleloop controller from the throughput manipulator to the bottleneck. However, i...