Merkel cell polyomavirus (MCPyV) is an oncogenic virus etiologically related to carcinoma and has been found in association with non-melanoma skin cancer (NMSC), controversial results. Little known about the role or influence of MCPyV development these tumors its prevalence various populations. To assess MCPyV`s Large T Antigen DNA cases NMSC, nested-PCR immunohistochemistry (IHC) were performe...

BACKGROUND Glycodelin A (GdA), also known as placental protein 14 (PP14), has been detected in endometrial, cervical and ovarian carcinoma cells. It is suspected to be a marker of human ovarian cancer tissues. MATERIALS AND METHODS We investigated serum, tissue and cyst fluid samples of patients with an ovarian carcinoma in contrast to patients with benign and malignant diseases such as uteri...

Abbreviations used in this paper: BM, basement membrane; BTB, blood–testis barrier; ChIP, chromatin immunoprecipitation; ES, ectoplasmic specialization; FSH, follicle-stimulating hormone; GCNA1, germ cell nuclear antigen 1; H&E, hematoxylin and eosin; Het, heterozygous; IF, immunofluorescence; IHC, immunohistochemistry; KO, knockout; LH, luteinizing hormone; MMP, matrix metalloproteinase; P, po...

Background: ​
 Pigmented lesion evaluation remains a challenging aspect of dermatology. The DermTech Melanoma Test (DMT) is non-invasive gene-expression test designed to rule-out melanoma. It consists the pigmented assay, which detects RNA products Long Intergenic Non-Coding 00518 (LINC00518) and Preferentially Expressed Antigen in (PRAME), an add-on assay for DNA promoter mutations telome...

Seborrheic keratosis (SK) is the most common benign cutaneous neoplasm. A subset shows increased p16 expression. Since SK shares several features with verruca vulgaris, e.g., increased p16 expression, human papillomaviruses (HPV) have been suggested as possible causal agents. However, a relevant association could not be established between HPV and SK. In the present study we aimed to investigat...

BACKGROUND Coxiella burnetii, Chlamydia abortus and Leptospira spp. are difficult to grow bacteria that play a role in bovine abortion, but their diagnosis is hampered by their obligate intracellular lifestyle (C. burnetii, C. abortus) or their lability (Leptospira spp.). Their importance is based on the contagious spread in food-producing animals, but also as zoonotic agents. In Switzerland, f...

Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0-3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA,...

BACKGROUND Small B-cell non-Hodgkins lymphoma (NHL) is difficult to be distinguished from non-neoplastic reactive processes using conventional haematoxylin-eosin (HE) staining due to different interpretations among pathologists with diagnosis based on morphologic features. Ancillary examinations such as immunohistochemical (IHC) staining are essential. However, negative or doubtful results are ...

Breast cancer is a significant cause of death in women. Estrogen receptor (ER) and progesterone receptor (PR) are important prognostic factors indicating higher recovery rate in the breast cancer patients. Currently, immunohistochemical (IHC) staining is a conventional method to identify expression of ER and PR. If a breast cancer patient expresses ER or PR, a chemotherapy with estrogen inhibit...

AIMS To evaluate the sensitivity and specificity of serological, immunohistochemical, and molecular methods in the diagnosis of post kala-azar dermal leishmaniasis (PKDL). METHODS Twenty five patients with confirmed PKDL and 25 controls were included in the study. G2D10, a monoclonal antibody against Leishmania, was used for the immunohistochemical (IHC) staining of lesion sections to visuali...