The behavior of rat transferrin has been investigated employing acrylamide gel electrophoresis and isoelectric focusing. In vitro trace labeling with iron chelates at 30 min was 93%-98% effective, whereas binding by simple ferric salts was reduced to 71%-76%. Complete and specific binding of 59FeSO4 by the iron binding sites of transferrin was demonstrated after in vitro or in vivo addition of ...

In previous studies, antitransferrin receptor antibody 42/6 inhibited growth of normal granulocyte/macrophage progenitors and some malignant myeloid cells. In these studies, leukemia cell lines cultured without serum and fresh leukemia cells were used to investigate the roles of Fe, transferrin receptors, and transferrin in leukemia cell growth, and mechanisms of 42/6 inhibition and resistance....

Several lines of recent evidence have suggested that transferrin plays a significant role in tissue interaction or morphogenesis at early stages of embryo development. In the present study, an anti-chicken transferrin antibody was produced and its basic characteristics were clarified as a basis for use in further studies. An antiserum termed Toraji 3 was raised against chicken transferrin and p...

Two unusual but similar cases of very high serum iron, extremely high transferrin concentrations (5.4 to 6.5 g/L), and increased transferrin saturation are reported. No anemia or microcytosis was observed. The ferritin concentration remained within the normal range, and no iron overload was observed. In one case, the in vivo half-life of 59Fe-labeled transferrin was shown to be prolonged (206 m...

Nutritional iron deficiency was produced experimentally by raising newly hatched chicks on an iron-deficient diet for several weeks. During this time, hematocrit and hemoglobin values declined, iron stores were depleted, and the circulating level of transferrin increased 2to 4-fold. The increase in serum transferrin was related to a similar increase in the rate of transferrin synthesis in liver...

We have studied the role of transferrin and the transferrin receptor in the uptake of "Ga by the human leukemic cell line HL60. In the absence of transit-mil, HL60 cells incorporated about 1% of the 67Ga dose over 6 h. The presence of transferrin increased cellular 67Ga uptake approxi mately 10-fold. Transferrin-mediated uptake of t7Ga was blocked by an anti-transferrin receptor monoclonal anti...

Norouziyan F, Shen WC, Hamm-Alvarez SF. Tyrphostin A8 stimulates a novel trafficking pathway of apically endocytosed transferrin through Rab11-enriched compartments in Caco-2 cells. Am J Physiol Cell Physiol 294: C7–C21, 2008. First published October 24, 2007; doi:10.1152/ajpcell.00372.2006.—The potential application of transferrin receptors as delivery vehicles for transport of macromolecular ...

1. Delanghe J, De Buyzere M. Carbohydrate deficient transferrin and forensic medicine. Clin Chim Acta 2009;406:1–7. 2. Delanghe JR, Helander A, Wielders JPM, Pekelharing JM, Roth HJ, Schellenberg F, et al. Development and multicenter evaluation of the N Latex CDT direct immunonephelometric assay for serum carbohydrate-deficient transferrin. Clin Chem 2007;53:1115–21. 3. Maenhout TM, De Buyzere ...

The gene for gonococcal transferrin-binding protein 1 (TBP1) was cloned behind an inducible promoter in Escherichia coli. The resultant strain was capable of binding human transferrin with the same specificity as that of the gonococcus. E. coli expressing TBP1 did not internalize transferrin-bound iron or grow on transferrin as a sole iron source.