Journal:
:molecular biology research communications
0
maral rahimzadeh
department of nanobiotechnology, school of biological sciences, tarbiat modares university, tehran, iran majid sadeghizadeh
department of molecular genetics, school of biological sciences, tarbiat modares university, tehran, iran farhood najafi
department of resin and additives, institute for color science and technology, tehran, iran shahriar arab
department of biophysics, school of biological sciences, tarbiat modares university, tehran, iran hamid mobasheri
laboratory of membrane biophysics, institute of biochemistry and biophysics, university of tehran, tehran, iran
cacl2 treatment followed by heat shock is the most common method for artificial transformation. here, the cells were transformed using cacl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. the bl21 strain of escherichia coli (e. coli) was being susceptible using cacl2 treatment. some cells were ...