When intact cells (HeLa S-3, mouse 3T3 fibroblasts, hen erythrocytes) are incubated with the rifamycin SV derivative AF/OI3, which is widely used as a potent inhibitor of mammalian DNA-dependent RNA polymerases, a rapid and extensive disintegration of the plasma membrane takes place. This cell disruption results, for example, in hemolysis of the erythrocytes at concentrations above 50 JLg/ml, i...

The antiperinuclear factor, an autoantibody specific for rheumatoid arthritis, was found in 51/63 (81%) patients with rheumatoid arthritis by indirect immunofluorescence on human buccal mucosa cells. The sensitivity of the antiperinuclear factor test was increased by pretreating the buccal mucosa cells with 0.5% Triton-X100. The specificity of the test for rheumatoid arthritis as compared with ...

The cellular localization of the origin of a-aminoadipate used in penicillin biosynthesis and the first enzymic step in Penicillium chrysogenum involved, &(aaminoadipy1)-L-cysteinyl-D-valine synthetase (ACVS), has been studied. Subcellular fractions were obtained from protoplasts of a high penicillin-producing strain upon lysis by Triton X100, and vacuoles purified from them. They were identifi...

Human Rhnu11 red blood cells fail to react with Rh antibodies, indicating that these cells are either devoid of Rh protein or, like other species, possess antigenically distinct variants. To determine whether Rhnull cells possess an Rh-like polypeptide, 32-kDa proteins from D--, rr, and Rhnull cells were labeled with the cysteine-specific probe, lZ61-labeled pyridyldithioethylamine. Size compar...

Calcium-dependent phosphorylation of endogenous substrate proteins in albino rabbit ciliary processes was studied by SDS-polyacrylamide gel electrophoresis and autoradiography. In the soluble fraction, a modest augmentation of phosphorylation was observed by Ca2+ alone and together with the additional activators, calmodulin (CAM) or phorbol myristate acetate (PMA). However, there was a greater ...

Thymosin beta4 is regarded as the main G-actin sequestering peptide in the cytoplasm of mammalian cells. It is also thought to be involved in cellular events like cancerogenesis, apoptosis, angiogenesis, blood coagulation and wound healing. Thymosin beta4 has been previously reported to localise intracellularly to the cytoplasm as detected by immunofluorescence. It can be selectively labelled a...

The present investigation was designed to study adhesion of calcium oxalate crystals on the surface of intact MDCK cells quantitatively, and to estimate the effects of glycosaminoglycans (GAGs) and cell injuries on these adhesions. Calcium oxalate monohydrate (COM) crystals adhere to the cell surface by an active force, and the attachment is in a time and concentration dependency with plateau. ...

A simple, selective and sensitive spectrophotometric method has been developed for the individual and simultaneous determination of Ti(IV) and Mo(VI) using salicylaldehyde p-hydroxybenzoylhydrazone (SAPHBH) in presence of Triton X100, without any prior separation. Beer’s law is obeyed between 0.15-2.0 and 0.20-2.51 μgmL concentration of Ti(IV) and Mo(VI) at 470 and 410 nm, respectively. The mol...

Nine substrates were investigated for phytase production by Penicillium funiculosum NRC467 fine particles of crushed fava bean has been selected as the best substrate using solid state fermentation (SSF). The SSF conditions were optimized by using one-variable-at-a-time (OVAT) strategy. Penicillin G has been used to enhance phytase production instead of the traditionally used Tween 80 and Trito...

Mutations of the Saccharomyces cerevisiae LOSl gene cause the accumulation of end matured introncontaining pre-tRNAs at elevated temperatures. In an effort to decipher the role of the LOSl protein in pretRNA splicing, we have analyzed the LOSl gene and its protein product. The LOSl gene is located on the left arm of chromosome XI and the order of genes in this area of the chromosome is . . . . ...